Product Information

Contents: Biotin anti-rat CD3
Catalog Number: 13-0030
Sizes: 50 ug, 100 ug, 500 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: eBioG4.18 (G4.18)
Isotype: Mouse IgG3, κ

Staining of Lou rat splenocytes with FITC anti-rat CD45R (HIS24) (cat. 11-0460) and staining buffer (autofluorescence) (left) or 0.125 μg of Biotin anti-rat CD3 (G4.18) (right) followed by Streptavidin-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0030	Affinity Purified anti-rat CD3	N/A	N/A	FC IHC IP WB
16-0030	Functional Grade* Purified anti-rat CD3	N/A	N/A	FA FC IHC WB
13-0030	Biotin anti-rat CD3	N/A	N/A	FC
11-0030	Fluorescein isothiocyanate (FITC) anti-rat CD3	488	518	FC
12-0030	Phycoerythrin (PE) anti-rat CD3	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioG4.18 monoclonal antibody recognizes the rat CD3 protein. CD3 is a critical component of the T-cell receptor (TCR) and is a marker of the T-cell lineage. CD3 is a complex of several subunits and, upon binding of a TCR ligand, participates in transduction of signals from the TCR to the nucleus which results in a variety of cellular responses including transcription of IL-2 and clonal expansion. in vitro, immobilized G4.18 monoclonal antibody has been demonstrated to activate T cells, while soluble G4.18 inhibited allogeneic mixed-lymphocyte proliferative responses and cell-mediated cytotoxicity to allogeneic target cells. in vivo, G4.18 was demonstrated to induce long-term specific tolerance to an organ allograft.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioG4.18 (G4.18) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioG4.18 (G4.18) antibody has been tested by flow cytometric analysis of rat splenocytes. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nicolls MR, Aversa GG, Pearce NW, Spinelli A, Berger MF, Gurley KE, Hall BM. Induction of long-term specific tolerance to allografts in rats by therapy with an anti-CD3-like monoclonal antibody. Transplantation. 1993 Mar;55(3):459-68. (G4.18, FC, IP, FA, PubMed)

Tran GT, Carter N, He XY, Spicer TS, Plain KM, Nicolls M, Hall BM, Hodgkinson SJ. Reversal of experimental allergic encephalomyelitis with non-mitogenic, non-depleting anti-CD3 mAb therapy with a preferential effect on T(h)1 cells that is augmented by IL-4. Int Immunol. 2001 Sep;13(9):1109-20. (G4.18, FC, FA, PubMed)

Upham JW, Strickland DH, Bilyk N, Robinson BW, Holt PG. Alveolar macrophages from humans and rodents selectively inhibit T-cell proliferation but permit T-cell activation and cytokine secretion. Immunology. 1995 Jan;84(1):142-7.

Brenan M, Rees DJ. Sequence analysis or rat integrin alphaE1 and alphaE2 subunits: tissue expression reveals phenotypic similarities between intraepithelial lymphocytes and dendritic cells in lymph. Eur J Immunol. 2000 Jun;30(6):1527-37.

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