Product Information

Contents: Biotin anti-human CD2 (LFA-2)
Catalog Number: 13-0029
Sizes: 25 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
Clone: RPA-2.10
Isotype: Mouse IgG1, κ
HLDA No.: IV T085

Staining of normal human peripheral blood cells with 0.125 μg of Biotin Mouse IgG1, K Iso Cntrl (cat. 13-4714) (open histogram) or 0.06 μg of Biotin anti-human CD2 (RPA-2.10) (colored histogram) followed by SAv-PE (cat. 12-4317). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-0029	Affinity Purified anti-human CD2 (LFA-2)	N/A	N/A	FA FC IH/F WB
16-0029	Functional Grade* Purified anti-human CD2 (LFA-2)	N/A	N/A	FC
13-0029	Biotin anti-human CD2 (LFA-2)	N/A	N/A	FC
11-0029	FITC anti-human CD2 (LFA-2)	488	518	FC
12-0029	PE anti-human CD2 (LFA-2)	488	575	FC
15-0029	Phycoerythrin-Cy5 (PE-Cy5) anti-human CD2 (LFA-2)	488	670	FC
17-0029	Allophycocyanin (APC) anti-human CD2 (LFA-2)	633	660	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RPA-2.10 monoclonal antibody reacts with human CD2, a 50 kDa cell surface receptor expressed by a majority of thymocytes, all mature T cells and subset of NK cells. CD2 is a ligand for CD58 in the human and is involved in adhesion and activation of T cells. RPA-2.10 blocks mixed lymphocyte reaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RPA-2.10 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The RPA-2.10 antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at less than or equal to 1 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Knapp, W., B. Dorken, et al. eds. (1989). Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press. New York.
Aversa GG, Bishop GA, Suranyi MG, Hall BM. (1987). RPA-2.10: an anti-CD2 monoclonal antibody that inhibits alloimmune responses and monitors T cell activation. Transplant Proc. 19:277-8

Related Products

Cat. 11-4317  Streptavidin-FITC (Fluorescein isothiocyanate)
Cat. 12-4317  Streptavidin-PE (Phycoerythrin)
Cat. 17-4317  Streptavidin Allophycocyanin (SA-APC)
Cat. 13-4714  Biotin Mouse IgG1, K Isotype Control