Contents: Phycoerythrin (PE) anti-human Perforin
Catalog Number: 12-9995
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 1B4
Isotype: Mouse IgG2b, κ
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Description
The 1B4 antibody reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granuels of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 1B4 antibody has been reported for use in intracellular flow cytometric analysis.
Applications Tested
This 1B4 antibody has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ortaldo JR, Winkler-Pickett RT, Nagashima K, Yagita H, Okumura K. 1992. Direct evidence for release of pore-forming protein during NK cellular lysis. J Leukoc Biol. 52(5):483-8.
Uellner R, Zvelebil MJ, Hopkins J, Jones J, MacDougall LK, Morgan BP, Podack E, Waterfield MD, Griffiths GM. 1997. Perforin is activated by a proteolytic cleavage during biosynthesis which reveals a phospholipid-binding C2 domain. EMBO J. 16(24):7287-96.
Nakata M, Kawasaki A, Azuma M, Tsuji K, Matsuda H, Shinkai Y, Yagita H, Okumura K. 1992. Expression of perforin and cytolytic potential of human peripheral blood lymphocyte subpopulations. Int Immunol. 4(9):1049-54.
Related Products
Cat.
12-4732 PE Mouse IgG2b Isotype Control
