Product Information

Contents: Phycoerythrin (PE) anti-human Perforin
Catalog Number: 12-9994
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: dG9 (delta G9)
Isotype: Mouse IgG2b, κ

Human PBMCs were surface-stained with APC anti-human CD8 (RPA-T8) (cat. 17-0088) and FITC anti-human CD56 (MEM188) (cat. 11-0569) then fixed and permeabilized and subsequently with PE anti-human Perforin (delta G9) or PE mouse IgG2b Iso Cntrl (cat. 12-4732). The density plot on the left demonstrates co-staining of CD56 and delta G9 while the right plot demonstrates co-staining of CD8 and delta G9. Quadrant lines demarcate the isotype control. Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9994	Affinity Purified anti-human Perforin	N/A	N/A	IHC IP
11-9994	Fluorescein isothiocyanate (FITC) anti-human Perforin	488	518	IC Flow IHC IP
12-9994	Phycoerythrin (PE) anti-human Perforin	488	575	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The dG9 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This dG9 (delta G9) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This dG9 (delta G9) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of human PBMCs. This can be used at 20 μl (0.125 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

M.J. Smyth, et al, (1990) J Exp Med 171: 1269-1281.
A. Hameed, et al, (1992) Am J Pathol 140: 1025-1030.
P.L. Jose, et al, (1994) J Immunol 148: 3354-3360.
B.C. Schlesinger & L. Cheng, (1994) Immunol 81: 291-295.
C.C. Liu, et al, (1995) Immunol Today 16: 194-201.

Related Products

Cat. 12-4732  PE Mouse IgG2b Isotype Control
Cat. 88-7095  Human Perforin ELISA Ready-SET-Go!
Cat. 88-7395  Human Perforin ELISPOT Ready-SET-Go!
Cat. 11-9995  **PLEASE SEE NEW CLONE, CAT# 11-9994 INSTEAD** Fluorescein isothiocyanate (FITC) anti-human Perforin (clone 1B4)
Cat. 12-9995  **PLEASE SEE NEW CLONE, CAT# 12-9994 INSTEAD** Phycoerythrin (PE) anti-human Perforin (clone 1B4)
Cat. 14-9995  Affinity Purified anti-human Perforin (clone 1B4)