Product Information

Contents: Phycoerythrin (PE) anti-human HLA-ABC
Catalog Number: 12-9983
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: W6/32
Isotype: Mouse IgG2a, κ

Staining of normal human peripheral blood cells with PE mouse IgG2a isotype control (cat. 12-4729) (open histogram) or PE W6/32 (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9983	Affinity Purified anti-human HLA-ABC	N/A	N/A	FC IH/F IP
16-9983	Functional Grade* Purified anti-human HLA-ABC	N/A	N/A	FC
13-9983	Biotin anti-human HLA-ABC	N/A	N/A	FC
11-9983	FITC anti-human HLA-ABC	488	518	FC
12-9983	PE anti-human HLA-ABC	488	575	FC
15-9983	PE-Cy5 anti-human HLA-ABC	488	670	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The W6/32 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The W6/32 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Barnstable, C. J., W. F. Bodmer, et al. 1978. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis. Cell 14(1): 9-20.

Koene G, Arts-Hilkes, et al.2004. High level of aneuploidy of chromosome 6 by FISH analysis of head and neck squamous cell carcinoma: limited applicability of LOH analysis to define HLA loss. Hum Immunol. 65(12):1455-62. (IHC frozen, PubMed)

Goldman-Wohl D, Ariel I, et al. 2001. A study of human leukocyte antigen G expression in hydatidiform moles. Am J Obstet Gynecol. 185(2):476-80. (IHC frozen, PubMed)

Stern PL, Beresford N, et al. 1987. Class I-like MHC molecules expressed by baboon placental syncytiotrophoblast. J Immunol. 138(4):1088-91. (baboon cross-reactivity, PubMed)

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Cat. 12-4724 Phycoerythrin (PE) Mouse IgG2a, K Isotype Control