PE anti-human HLA-ABC antibody: r-phycoerythin (r-PE) conjugate

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Contents: Phycoerythrin (PE) anti-human HLA-ABC
Catalog Number: 12-9983
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: W6/32
Isotype: Mouse IgG2a, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-9983-71	25 tests	$100
12-9983-73	100 tests	$220

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with PE mouse IgG2a isotype control (cat. 12-4729) (open histogram) or PE W6/32 (colored histogram). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9983	Affinity Purified anti-human HLA-ABC	N/A	N/A	FC IH/F IP
16-9983	Functional Grade* Purified anti-human HLA-ABC	N/A	N/A	FC
13-9983	Biotin anti-human HLA-ABC	N/A	N/A	FC
11-9983	FITC anti-human HLA-ABC	488	518	FC
12-9983	PE anti-human HLA-ABC	488	575	FC
15-9983	PE-Cy5 anti-human HLA-ABC	488	670	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The W6/32 monoclonal antibody reacts with the human major histocompatibility complex (MHC) class I, HLA-A, B, C. MHC class I antigens associated with β2-microglobulin are expressed by all human nucleated cells and are central in cell-mediated immune response and tumor surveillance. W6/32 mAb recognizes a non-polymorphic epitope shared among products of the HLA-A, B, and C loci and immunoprecipitates both 43 kDa and 11-12 kDa chains.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The W6/32 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The W6/32 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25μg) per 100μl blood (or per 1 million cells in 100 μl total staining volume).

References

Barnstable, C. J., W. F. Bodmer, et al. (1978). "Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis." Cell 14(1): 9-20.

Related Products

Cat. 12-4724 Phycoerythrin (PE) Mouse IgG2a, K Isotype Control