Product Information

Contents: Phycoerythrin (PE) anti-mouse PD-1 (CD279, PD1)
Catalog Number: 12-9981
Sizes: 50 ug, 100 ug, 200 ug
Formulation: aqueous buffer, 0.09% sodium azide, contains stabilizer if necessary
Storage Conditions: Store at 2-8°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RMP1-30
Isotype: Rat IgG2b, κ

Surface staining of ConA stimulated splenocytes with anti-mouse PD-1 (RMP1-30) 0.25μg FITC (left), and 0.5μg PE (right). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9981	Affinity Purified anti-mouse PD-1 (CD279, PD1)	N/A	N/A	FC
16-9981	Functional Grade* Purified anti-mouse PD-1 (CD279, PD1)	N/A	N/A	FC
13-9981	Biotin anti-mouse PD-1 (CD279, PD1)	N/A	N/A	FC
11-9981	Fluorescein (FITC) anti-mouse PD-1 (CD279, PD1)	488	518	FC
12-9981	Phycoerythrin (PE) anti-mouse PD-1 (CD279, PD1)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The RMP1-30 antibody reacts with mouse PD-1 (programmed death-1), a 55 kDa member of the Ig superfamily. PD-1 contains the immunoreceptor tyrosine-based inhibitory motif (ITIM) and plays a key role in peripheral tolerance and autoimmune disease in mice. PD-1 is expressed mainly on activated T and B lymphocytes. Two novel B7 Family members have been identified as PD-1 ligands, PD-L1 (B7-H1) and PD-L2 (B7-DC). Evidence reported to date suggests overlapping functions for these ligands and their constitutive expression on some normal tissues and upregulation on activated antigen-presenting cells. RMP1–30 does not block the binding of either B7-H1-Ig or B7-DC-Ig to PD-1 transfectants.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RMP1-30 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This RMP1-30 antibody has been tested by flow cytometric analysis of mouse ConA-activated spleen cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Matsumoto K, Inoue H., et al. 2004. B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism. J Immunol. 172(4):2530-41.
Agata, Y., A. Kawasaki, et al. (1996). Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes. Int Immunol 8(5): 765-72.
Nishimura, H., Y. Agata, et al. (1996). Developmentally regulated expression of the PD-1 protein on the surface of double-negative (CD4-CD8-) thymocytes. Int Immunol 8(5): 773-80.
Nishimura, H., T. Okazaki, et al. (2001). Autoimmune dilated cardiomyopathy in PD-1 receptor-deficient mice. Science 291(5502): 319-22.
Freeman, G. J., A. J. Long, et al. (2000). Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med 192(7): 1027-34.

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