Contents: Phycoerythrin (PE) anti-mouse B7-DC (B7DC, PD-L2, PDL-2, PDL2)
Catalog Number: 12-9972
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 122
Isotype: Rat IgG2a, κ
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Staining of in vitro BM-derived dendritic cells from BALB/c with 0.125 μg PE Rat IgG2a Iso Cntrl (cat. 12-4321) (open histogram) or 0.06 μg PE anti-mouse B7-DC (122)(colored histogram). Total viable cells were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-9972 |
Affinity Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) |
N/A |
N/A |
FC
|
| 13-9972 |
Biotin anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) |
N/A |
N/A |
FC
|
| 11-9972 |
Fluorescein (FITC) anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) |
488 |
518 |
FC
|
| 12-9972 |
Phycoerythrin (PE) anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) |
488 |
575 |
FC
|
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific BlueŽ and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The 122 monoclonal antibody reacts with mouse B7-DC, also known as PD-L2. B7-DC, a recently identified member of the B7 family, has a predicted molecular weight of approximately 25 kDa and belongs to the Ig superfamily. The mouse B7-DC has a short cytoplasmic tail (4aa). B7-DC is primarily expressed by subpopulations of dendritic cells and monocytes/macrophages in the mouse. Although B7-DC has structural and sequence similarities to the B7 family, it does not bind CD28/CTLA-4, rather it is a ligand for PD-1. The interactions between PD-1 and B7-DC/PD-L2 have been reported to be involved in costimulation or suppression of T cell proliferation depending on state of cellular activation. 122 has been demonstrated to block binding of TY25 (Cat. No.
14-5986), another mAb specific for mouse B7-DC.
Applications Reported
For research use only, not for diagnostic or therapeutic use. 122 has been reported for use in flow cytometric analysis.
Applications Tested
The 122 antibody has been tested by flow cytometric analysis of mouse dendritic cell suspensions and mouse B7-DC transfected cells. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ishida M, Iwai Y, Tanaka Y, Okazaki T, Freeman GJ, Minato N, Honjo T. 2002. Differential expression of PD-L1 and PD-L2, ligands for an inhibitory receptor PD-1, in the cells of lymphohematopoietic tissues. Immunol Lett. 84(1):57-62. Carter L, Fouser LA, Jussif J, Fitz L, Deng B, Wood CR, Collins M, Honjo T, Freeman GJ, Carreno BM. 2002. PD-1:PD-L inhibitory pathway affects both CD4(+) and CD8(+) T cells and is overcome by IL-2. Eur J Immunol. 32(3):634-43.
Related Products
Cat. 12-4321 PE Rat IgG2a Isotype ControlCat. 12-5986 PE anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)Cat. 13-5986 Biotin anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)Cat. 14-5986 Affinity Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)Cat. 16-5986 Functional Grade Purified anti-mouse B7-DC (CD273, B7DC, PD-L2, PDL-2, PDL2) (clone TY25)
Mouse Cellular Antigens Chart