Product Information

Contents: Phycoerythrin (PE) anti-human HLA-E
Catalog Number: 12-9953
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 3D12HLA-E
Isotype: Mouse IgG1

Staining of normal human peripheral blood cells with FITC anti-human HLA-ABC (W6/32) (cat. 11-9983) and 0.5 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or PE anti-human HLA-E (3D12HLA-E) (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9953	Affinity Purified anti-human HLA-E	N/A	N/A	FC IP WB
12-9953	Phycoerythrin (PE) anti-human HLA-E	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 3D12 (HLA-E) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This 3D12 (HLA-E) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.

References

Wooden SL, Kalb SR, Cotter RJ, Soloski MJ. Cutting edge: HLA-E binds a peptide derived from the ATP-binding cassette transporter multidrug resistance-associated protein 7 and inhibits NK cell-mediated lysis. J Immunol. 2005 Aug 1;175(3):1383-7.(3D12 HLA-E, FC, PubMed)

Strong RK, Holmes MA, Li P, Braun L, Lee N, Geraghty DE. HLA-E allelic variants. Correlating differential expression, peptide affinities, crystal structures, and thermal stabilities. J Biol Chem. 2003 Feb 14;278(7):5082-90. Epub 2002 Oct 30.(3D12 HLA-E, FC, ELISA, PubMed)

Lee N, Llano M, Carretero M, Ishitani A, Navarro F, López-Botet M, Geraghty DE. HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A. Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5199-204. (3D12 HLA-E, FC, FA, PubMed)

Related Products

Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control