Staining of normal human peripheral blood cells with Anti-Human HLA-ABC FITC (cat. 11-9983) and Mouse IgG1 κ Isotype Control PE (cat. 12-4714) (left) or Anti-Human HLA-E PE (right). Cells in the lymphocyte gate were used for analysis.
Contents: Anti-Human HLA-E PE Catalog Number: 12-9953 Concentration: 5 μl (0.5 μg)/test Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: 3D12HLA-E Host/Isotype: Mouse IgG1
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12-9953-41
25 tests
12-9953-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 3D12 (HLA-E) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 3D12 (HLA-E) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 μl (0.5 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Wooden SL, Kalb SR, Cotter RJ, Soloski MJ. Cutting edge: HLA-E binds a peptide derived from the ATP-binding cassette transporter multidrug resistance-associated protein 7 and inhibits NK cell-mediated lysis. J Immunol. 2005 Aug 1;175(3):1383-7.(3D12 HLA-E, FC, PubMed)
Strong RK, Holmes MA, Li P, Braun L, Lee N, Geraghty DE. HLA-E allelic variants. Correlating differential expression, peptide affinities, crystal structures, and thermal stabilities.
J Biol Chem. 2003 Feb 14;278(7):5082-90. Epub 2002 Oct 30.(3D12 HLA-E, FC, ELISA, PubMed)
Lee N, Llano M, Carretero M, Ishitani A, Navarro F, López-Botet M, Geraghty DE. HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A.
Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5199-204. (3D12 HLA-E, FC, FA, PubMed)
