Staining of normal human peripheral blood cells with FITC anti-human HLA-ABC (W6/32) (cat. 11-9983) and 0.5 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or PE anti-human HLA-E (3D12HLA-E) (right). Cells in the lymphocyte gate were used for analysis.
Contents: Phycoerythrin (PE) anti-human HLA-E Catalog Number: 12-9953 Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization Clone: 3D12HLA-E Host/Isotype: Mouse IgG1 Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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12-9953-41
25 tests
12-9953-42
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The monoclonal antibody 3D12 (HLA-E) recognizes human HLA-E, a member of the Human Leukocyte Antigen family. HLA-E is a MHC Class IB member, which has a specialized role in NK cell recognition. Binding of a restricted set of peptides derived from other Class I leader sequences to the HLA-E and B2M (beta2 microglobulin) complex leads to interaction with NKG2A on NK cells. This binding protects the HLA-G-expressing cell from NK cell killing. HLA-E expression is ubiquitous.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 3D12 (HLA-E) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This 3D12 (HLA-E) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. Please refer to the antibody vial for the recommended test volume, which contains 0.5 μg, to use for one million cells in a 100 μl total staining volume.
References
Wooden SL, Kalb SR, Cotter RJ, Soloski MJ. Cutting edge: HLA-E binds a peptide derived from the ATP-binding cassette transporter multidrug resistance-associated protein 7 and inhibits NK cell-mediated lysis. J Immunol. 2005 Aug 1;175(3):1383-7.(3D12 HLA-E, FC, PubMed)
Strong RK, Holmes MA, Li P, Braun L, Lee N, Geraghty DE. HLA-E allelic variants. Correlating differential expression, peptide affinities, crystal structures, and thermal stabilities.
J Biol Chem. 2003 Feb 14;278(7):5082-90. Epub 2002 Oct 30.(3D12 HLA-E, FC, ELISA, PubMed)
Lee N, Llano M, Carretero M, Ishitani A, Navarro F, López-Botet M, Geraghty DE. HLA-E is a major ligand for the natural killer inhibitory receptor CD94/NKG2A.
Proc Natl Acad Sci U S A. 1998 Apr 28;95(9):5199-204. (3D12 HLA-E, FC, FA, PubMed)
