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Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq. DescriptionThe C398.4A mAb reacts with ICOS (Inducible COStimulatory molecule), also known as H4/CRP-1/AILIM, and cross-reacts with mouse and rat ICOS. ICOS is a T cell specific activation molecule and a third member of the CD28/CTLA-4 family. A dimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1, is thought to play a protective role in inflammatory autoimmune diseases and be involved in the development of Th2 cells. C398.4A is reported to display strong costimulation of proliferation of T cell clones, intermediate costimulation on activated T cells, and weak costimulation on fresh resting T cells. It is a good costimulator of IL-10 production especially from pre-activated cells that express high level of H4/ICOS. The epitope recognized by C398.4A is different from that bound by the ICOSL (i.e. C398.4A does not block binding of ICOSL). Applications ReportedFor research use only, not for diagnostic or therapeutic use. The C398.4A antibody has been reported for use in flow cytometric analysis. Applications TestedThis C398.4A antibody has been tested by flow cytometric analysis of mouse ConA-activated (2 days) spleen cell suspensions. This can be used at less than or equal to 0.125 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. References
Lucia, M. B., D. Buonfiglio, et al. 2000. Expression of the novel T cell activation molecule hpH4 in HIV-infected patients: correlation with disease status. AIDS Res Hum Retroviruses 16(6): 549-57. Buonfiglio, D., M. Bragardo, et al. 1999. Characterization of a novel human surface molecule selectively expressed by mature thymocytes, activated T cells and subsets of T cell lymphomas. Eur J Immunol 29(9): 2863-74. Redoglia, V., U. Dianzani, et al. 1996. Characterization of H4: a mouse T lymphocyte activation molecule functionally associated with the CD3/T cell receptor. Eur J Immunol 26(11): 2781-9. Arimura Y, Shiroki F, Kuwahara S, Kato H, Dianzani U, Uchiyama T, Yagi J. Akt is a neutral amplifier for Th cell differentiation. J Biol Chem. 2004 Mar 19;279(12):11408-16. Epub 2004 Jan 5. [activating] Related ProductsCat. 12-4888 Phycoerythrin (PE) Armenian Hamster IgG Isotype Control (clone eBio299Arm) Cat. 12-9940 PE anti-mouse ICOS (CD278) (clone 15F9) Cat. 13-9940 Biotin anti-mouse ICOS (CD278) (clone 15F9) Cat. 14-9940 Affinity Purified anti-mouse ICOS (CD278) (clone 15F9) Cat. 15-9940 PE-Cy5 anti-mouse ICOS (CD278) (clone 15F9) Cat. 12-9942 PE anti-mouse ICOS (CD278) (clone 7E.17G9) Cat. 13-9942 Biotin anti-mouse ICOS (CD278) (clone 7E.17G9) Cat. 14-9942 Affinity Purified anti-mouse ICOS (CD278) (clone 7E.17G9) Cat. 15-9942 PE-Cy5 anti-mouse ICOS (CD278) (clone 7E.17G9) Cat. 16-9942 Functional Grade Purified anti-mouse ICOS (CD278) (clone 7E.17G9) Cat. 11-9948 FITC anti-human ICOS (CD278) (clone ISA-3) Cat. 12-9948 Phycoerythrin (PE) anti-human ICOS (CD278) (clone ISA-3) Cat. 13-9948 Biotin anti-human ICOS (CD278) (clone ISA-3) Cat. 14-9948 Affinity Purified anti-human ICOS (CD278) (clone ISA-3) Cat. 16-9948 Functional Grade Purified anti-human ICOS (CD278) (clone ISA-3) |
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Mouse Cellular Antigens Chart