Product Information

Contents: Phycoerythrin (PE) anti-human ZAP-70 (ZAP70)
Catalog Number: 12-9945
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 2F3.2
Isotype: Mouse IgG2a

Intracellular staining of normal human peripheral blood cells with FITC anti-human CD4 (OKT4) (cat. 11-0048) and 0.25 μg of PE Mouse IgG2a, K Iso Cntrl (cat. 12-4724) (left) or PE anti-human ZAP-70 (2F3.2) (right) using FoxP3 buffers (cat. 00-5523). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications

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Description

This 2F3.2 monoclonal antibody reacts with human zeta-chain associated protein 70 (ZAP-70), a cytoplasmic tyrosine kinase expressed in T cells, natural killer cells, as well as in developing and some mature B cell subsets. Upon T cell activation, ZAP-70 is recruited to the T cell receptor (TCR) and subsequently phosphorylates signaling molecules such as LAT and SLP-76. In addition to its role in TCR signaling, ZAP-70 has been implicated in thymocyte development.

ZAP-70 is also a surrogate biomarker for determining chronic lymphocytic leukemia (CLL) prognosis. Expression of this kinase is used to indicate the somatic mutational status of the immunoglobulin variable heavy chain gene (IgVH). Genomic studies revealed that ZAP-70 gene expression correlates with an unmutated IgVH gene. This clone stains similarly to 1E7.2 and, based on antibody blocking experiments, recognizes a different epitope.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This 2F3.2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This 2F3.2 antibody has been pre-titrated and tested by intracellular flow cytometric analysis on normal human peripheral blood cells. This can be used at 5 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Palacios EH, Weiss A. Distinct roles for Syk and ZAP-70 during early thymocyte development.J Exp Med. 2007 Jul 9;204(7):1703-15.

Letestu, R, et al. Evaluation of ZAP-70 expression by flow cytometry in chronic lymphocytic leukemia: A multicentric international harmonization process. Cytometry Part B (Clinical Cytometry) 2006 70B:309-314.

Rassenti LZ, Huynh L, Toy TL, Chen L, Keating MJ, Gribben JG, Neuberg DS, Flinn IW, Rai KR, Byrd JC, Kay NE, Greaves A, Weiss A, Kipps TJ. ZAP-70 compared with immunoglobulin heavy-chain gene mutation status as a predictor of disease progression in chronic lymphocytic leukemia. N Engl J Med. 2004 Aug 26;351(9):893-901.

Related Products

Cat. 12-4724  Phycoerythrin (PE) Mouse IgG2a, K Isotype Control
Cat. 00-5523  eBioscience Foxp3 Staining Buffer Set
Cat. 12-6695  PE anti-mouse/human ZAP-70 (ZAP70) (clone 1E7.2)