Product Information

Contents: Phycoerythrin (PE) anti-mouse ICOS (CD278)
Catalog Number: 12-9942
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 7E.17G9
Isotype: Rat IgG2b, κ

Staining of unstimulated (left) and 3 day ConA activated (right) BALB/c splenocytes with 0.06 µg of PE rat IgG2b isotype control (cat.12-4331) (open histogram) or 0.06 µg of PE 7E.17G9 (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9942	Affinity Purified anti-mouse ICOS (CD278)	N/A	N/A	FC
16-9942	Functional Grade* Purified anti-mouse ICOS (CD278)	N/A	N/A	FC
13-9942	Biotin anti-mouse ICOS (CD278)	N/A	N/A	FC
12-9942	PE anti-mouse ICOS (CD278)	488	575	FC
15-9942	PE-Cy5 anti-mouse ICOS (CD278)	488	670	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 7E.17G9 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and ConA activated (3 day) mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Akbari O, Freeman GJ, Meyer EH, Greenfield EA, Chang TT, Sharpe AH, Berry G, DeKruyff RH, Umetsu DT. 2002. Antigen-specific regulatory T cells develop via the ICOS-ICOS-ligand pathway and inhibit allergen-induced airway hyperreactivity. Nat Med. 8:1024-32.
McAdam AJ, Greenwald RJ, Levin MA, Chernova T, Malenkovich N, Ling V, Freeman GJ, Sharpe AH. 2001. ICOS is critical for CD40-mediated antibody class switching. Nature. 409:102-5.
McAdam AJ, Chang TT, Lumelsky AE, Greenfield EA, Boussiotis VA, Duke-Cohan JS, Chernova T, Malenkovich N, Jabs C, Kuchroo VK, Ling V, Collins M, Sharpe AH, Freeman GJ. 2000. Mouse inducible costimulatory molecule (ICOS) expression is enhanced by CD28 costimulation and regulates differentiation of CD4+ T cells. J Immunol. 165:5035-40.

Related Products

Cat. 12-4031  Phycoerythrin (PE) Rat IgG2b Isotype Control
Cat. 12-9940  PE anti-mouse ICOS (CD278) (clone 15F9)
Cat. 13-9940  Biotin anti-mouse ICOS (CD278) (clone 15F9)
Cat. 14-9940  Affinity Purified anti-mouse ICOS (CD278) (clone 15F9)
Cat. 15-9940  PE-Cy5 anti-mouse ICOS (CD278) (clone 15F9)
Cat. 11-9949  FITC anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 12-9949  PE anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 13-9949  Biotin anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 14-9949  Affinity Purified anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 15-9949  PE-Cy5 anti-mouse/rat ICOS (CD278) (clone C398.4A)
Cat. 16-9949  Functional Grade Purified anti-mouse/rat ICOS (CD278) (clone C398.4A)