Staining of unstimulated (left) and 3 day ConA-stimulated (right) BALB/c splenocytes with 0.06 µg of Rat IgG2b κ Isotype Control PE (cat. 12-4031) (open histogram) or 0.06 µg of Anti-Mouse CD278 (ICOS) PE (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Mouse CD278 (ICOS) PE Catalog Number: 12-9942 Concentration: 0.2 mg/ml Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: 7E.17G9 Host/Isotype: Rat IgG2b, κ
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Size
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12-9942-81
50 ug
12-9942-82
100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 7E.17G9 antibody has been reported for use in flow cytometric analysis.
Applications Tested
The 7E.17G9 antibody has been tested by flow cytometric analysis of unstimulated and ConA activated (3 day) mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Akbari O, Freeman GJ, Meyer EH, Greenfield EA, Chang TT, Sharpe AH, Berry G, DeKruyff RH, Umetsu DT. 2002. Antigen-specific regulatory T cells develop via the ICOS-ICOS-ligand pathway and inhibit allergen-induced airway hyperreactivity. Nat Med. 8:1024-32. McAdam AJ, Greenwald RJ, Levin MA, Chernova T, Malenkovich N, Ling V, Freeman GJ, Sharpe AH. 2001. ICOS is critical for CD40-mediated antibody class switching. Nature. 409:102-5. McAdam AJ, Chang TT, Lumelsky AE, Greenfield EA, Boussiotis VA, Duke-Cohan JS, Chernova T, Malenkovich N, Jabs C, Kuchroo VK, Ling V, Collins M, Sharpe AH, Freeman GJ. 2000. Mouse inducible costimulatory molecule (ICOS) expression is enhanced by CD28 costimulation and regulates differentiation of CD4+ T cells. J Immunol. 165:5035-40.
