Product Information

Contents: Phycoerythrin (PE) conjugated anti-mouse MD-1
Catalog Number: 12-9931
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
LIGHT-SENSITIVE MATERIAL
DO NOT FREEZE
Clone: MD113
Isotype: Rat IgG2b, κ

Staining of C57Bl/6 splenocytes with 0.25 µg PE rat IgG2b isotype control (cat.12-4331) (open histogram) or 0.25 µg PE anti-mouse MD-1 (MD113)(colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9931	Affinity Purified anti-mouse MD-1 (MD1; Toll-like Receptor/ TLR Family)	N/A	N/A	FC
16-9931	Functional Grade* Purified anti-mouse MD-1 (MD1; Toll-like Receptor/ TLR Family)	N/A	N/A	FC
13-9931	Biotin anti-mouse MD-1 (MD1; Toll-like Receptor/ TLR Family)	N/A	N/A	FC
12-9931	PE anti-mouse MD-1 (MD1; Toll-like Receptor/ TLR Family)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MD113 monoclonal antibody reacts with mouse MD-1, a 28 kDa molecule, which physically associates with the extracellular portion of RP105 (CD180). MD-1 is expressed by mature B cells, monocytes/macrophages and dendritic cells. The coexpression of MD-1 is indispensable for cell surface expression of RP105 and LPS recognition and signaling. Several monoclonal antibodies to mouse MD-1, including MD14 and MD113, have been reported to be antagonistic for LPS-induced B cell proliferation and CD86 upregulation, while the anti-mouse CD180 (clone RP/14, Cat. No. 16-1801) is reported to exert potent mitogenic effect. MD113 has been reported to suppress LPS-induced B cell responses to a higher degree than MD14 (Cat. No. 14-9921).

Applications Reported

For research use only, not for diagnostic or therapeutic use. MD113 has been reported for use in flow cytometric analysis.

Applications Tested

The MD113 antibody has been tested by flow cytometric analysis of mouse spleen cell suspensions and can be used at less than or equal to 0.5 µg per million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Nagai, Y., R. Shimazu, et al. 2002. Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide. Blood 99(5):1699-705.
Miyake, K., R. Shimazu, et al. 1998. Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression. J Immunol 161(3): 1348-53.
Miyake, K., H. Ogata, et al. 2000. Innate recognition of lipopolysaccharide by Toll-like receptor 4/MD-2 and RP105/MD-1. J Endotoxin Res 6(5):389-91.
Miura, Y., R. Shimazu, et al. 1998. RP105 is associated with MD-1 and transmits an activation signal in human B cells. Blood 92(8):2815-22.
Gorczynski, R. M., Z. Chen, et al. 2000. Regulation of gene expression of murine MD-1 regulates subsequent T cell activation and cytokine production. J Immunol 165(4):1925-32.
Ogata, H., I. Su, et al. 2000. The toll-like receptor protein RP105 regulates lipopolysaccharide signaling in B cells. J Exp Med 192(1):23-9.

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