Product Information

Contents: Phycoerythrin (PE) anti-human Trail (CD253)
Catalog Number: 12-9927
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RIK-2
Isotype: Mouse IgG1, κ

Staining of non-transfected (left) and human TRAIL transfected 2PK3 cells (right) with staining buffer (autofluorescence) (open histogram) or PE RIK-2 (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-9927	PE anti-human Trail (CD253)	488	575	FC
13-9927	Biotin anti-human Trail (CD253)	N/A	N/A	FC
14-9927	Affinity Purified anti-human Trail (CD253)	N/A	N/A	FA FC
16-9927	Functional Grade* Purified anti-human Trail (CD253)	N/A	N/A	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RIK-2 monoclonal antibody reacts with human TNF-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily. TRAIL is expressed by normal human T, B and NK cells and by several human tumor lines. Interaction of TRAIL with its ligand, Apo-2, induces apoptosis. RIK-2 blocks TRAIL-induced apoptosis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The RIK-2 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The RIK-2 antibody has been pre-titrated and tested by flow cytometric analysis of human TRAIL transfected cells. This can be used at 20 μl per million cells in a 100 μl total staining volume.

References

Kayagaki, N., N. Yamaguchi, et al. (1999). "Expression and function of TNF-related apoptosis-inducing ligand on murine activated NK cells." J Immunol 163(4): 1906-13.
Kaplan, M. J., D. Ray, et al. (2000). "TRAIL (Apo2 ligand) and TWEAK (Apo3 ligand) mediate CD4+ T cell killing of antigen-presenting macrophages [In Process Citation]." J Immunol 164(6): 2897-904.
Kayagaki, N., N. Yamaguchi, et al. (1999). "Involvement of TNF-related apoptosis-inducing ligand in human CD4+ T cell-mediated cytotoxicity." J Immunol 162(5): 2639-47.

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