Anti-Human CD282 (TLR2) PE

Also known as: TLR-2, toll-like receptor 2

RUO: For Research Use Only

PE anti-human TLR2 (CD282, TLR2, TLR-2) antibody: r-phycoerythin, PE anti-human toll-like receptor 2

Staining of normal human peripheral blood cells with 2 µg of Mouse IgG2a κ Isotype Control PE (cat. 12-4724) (open histogram) or Anti-Human CD282 (TLR2) PE (filled histogram). Cells in the monocyte gate were used for analysis.

Contents: Anti-Human CD282 (TLR2) PE
Catalog Number: 12-9922
Concentration: 5 μl (1 μg)/test
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material.
Clone: TL2.1
Host/Isotype: Mouse IgG2a, κ

Product Options
Cat. No.	Size	Price	Add Qty to Cart
12-9922-41	25 tests	To view relevant pricing, please select your country and resume browsing.
12-9922-42	100 tests

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
12-4724	Mouse IgG2a K Isotype Control PE
14-8185	B18R Recombinant Protein
14-9029	Anti-Human CD282 (TLR2) Purified (TL2.3)
34-8185	B18R Recombinant Protein Carrier-Free

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
11-9922	Anti-Human CD282 (TLR2) FITC	488	518	FC	RUO
12-9922	Anti-Human CD282 (TLR2) PE	488	575	FC	RUO
13-9922	Anti-Human CD282 (TLR2) Biotin	N/A	N/A	FC	RUO
14-9922	Anti-Human CD282 (TLR2) Purified	N/A	N/A	FC IHC IP WB	RUO
16-9922	Anti-Human CD282 (TLR2) Functional Grade* Purified	N/A	N/A	FA FC	RUO
17-9922	Anti-Human CD282 (TLR2) APC	633	660	FC	RUO
51-9922	Anti-Human CD282 (TLR2) Alexa Fluor® 647 (To Be Discontinued. Refer to Alternative Format: APC, cat. 17-9922)	633	668	FC	RUO
53-9922	Anti-Human CD282 (TLR2) Alexa Fluor® 488	488	519	FC	RUO
56-9922	Anti-Human CD282 (TLR2) Alexa Fluor® 700	633	723	FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.

Description

The TL2.1 monoclonal antibody reacts with human Toll-like receptor 2 (TLR2). To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR2 is expressed by peripheral blood monocytes and is responsible for distinguishing different pathogens. TL2.1, a blocking antibody, has been used to study the role of TLR-2 as a pattern recognition receptor in microbial lipoprotein/lipopeptide induced cytokine production from human peripheral blood mononuclear cells. TL2.1 has been reported to immunoprecipitate human TLR2 (~90 kDa) from PBMC and HMEC.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The TL2.1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The TL2.1 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 5 μl (1 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test.

References

Burgener IA, Jungi TW. Antibodies specific for human or murine Toll-like receptors detect canine leukocytes by flow cytometry. Vet Immunol Immunopathol. 2008 Jul 15;124(1-2):184-91. (TL2.1, canine cross-reactivity, PubMed)

Do JE, Kwon SY, Park S, Lee ES. Effects of vitamin D on expression of Toll-like receptors of monocytes from patients with Behcet's disease. Rheumatology (Oxford). 2008 Jun;47(6):840-8. (TL2.1, FA, PubMed)

Mandron M, Ariès MF, Boralevi F, Martin H, Charveron M, Taieb A, Davrinche C. Age-related differences in sensitivity of peripheral blood monocytes to lipopolysaccharide and Staphylococcus aureus toxin B in atopic dermatitis. J Invest Dermatol. 2008 Apr;128(4):882-9. (TL2.1, FA, PubMed)

Bonnefont-Rebeix C, Marchal T, Bernaud J, Pin JJ, Leroux C, Lebecque S, Chabanne L, Rigal D. Toll-like receptor 3 (TLR3): a new marker of canine monocytes-derived dendritic cells (cMo-DC). Vet Immunol Immunopathol. 2007 Jul 15;118(1-2):134-9. (TL2.1, canine cross-reactivity, PubMed)

Chan G, Guilbert LJ. Ultraviolet-inactivated human cytomegalovirus induces placental syncytiotrophoblast apoptosis in a Toll-like receptor-2 and tumour necrosis factor-alpha dependent manner. J Pathol. 2006 Sep;210(1):111-20. (TL2.1, WB and IHC frozen, PubMed)

Cook EB, Stahl JL, Esnault S, Barney NP, Graziano FM. Toll-like receptor 2 expression on human conjunctival epithelial cells: a pathway for Staphylococcus aureus involvement in chronic ocular proinflammatory responses. Ann Allergy Asthma Immunol. 2005 Apr;94(4):486-97.(TL2.1, FA, PubMed)

Ueta M, Nochi T, Jang MH, Park EJ, Igarashi O, Hino A, Kawasaki S, Shikina T, Hiroi T, Kinoshita S, Kiyono H. Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium. J Immunol. 2004 Sep 1;173(5):3337-47. (TL2.1, IHC frozen, PubMed)

Iwahashi M, Yamamura M, Aita T, Okamoto A, Ueno A, Ogawa N, Akashi S, Miyake K, Godowski PJ, Makino H. Expression of Toll-like receptor 2 on CD16+ blood monocytes and synovial tissue macrophages in rheumatoid arthritis. Arthritis Rheum. 2004 May;50(5):1457-67. (TL2.1, FA, PubMed)

Coleman JL, Benach JL. The urokinase receptor can be induced by Borrelia burgdorferi through receptors of the innate immune system. Infect Immun. 2003 Oct;71(10):5556-64. (TL2.1, FA, PubMed)

Mori Y, Yoshimura A, Ukai T, Lien E, Espevik T, Hara Y. Immunohistochemical localization of Toll-like receptors 2 and 4 in gingival tissue from patients with periodontitis. Oral Microbiol Immunol. 2003 Feb;18(1):54-8. (TL2.1, IHC paraffin, PubMed)

Droemann D, Goldmann T, Branscheid D, Clark R, Dalhoff K, Zabel P, Vollmer E. Toll-like receptor 2 is expressed by alveolar epithelial cells type II and macrophages in the human lung. Histochem Cell Biol. 2003 Feb;119(2):103-8. (TL2.1, IHC paraffin, PubMed)

Faure E, Thomas L, Xu H, Medvedev A, Equils O, Arditi M. Bacterial lipopolysaccharide and IFN-gamma induce Toll-like receptor 2 and Toll-like receptor 4 expression in human endothelial cells: role of NF-kappaB activation. J Immunol. 2001 Feb 1;166(3):2018-24. (TL2.1, WB and IHC frozen, PubMed)

Flo TH, Halaas O, Lien E, Ryan L, Teti G, Golenbock DT, Sundan A, Espevik T. Human toll-like receptor 2 mediates monocyte activation by Listeria monocytogenes, but not by group B streptococci or lipopolysaccharide. J Immunol. 2000 Feb 15;164(4):2064-9. (TL2.1, Immunoprecipitation)

Lien E, Sellati TJ, Yoshimura A, Flo TH, Rawadi G, Finberg RW, Carroll JD, Espevik T, Ingalls RR, Radolf JD, Golenbock DT. Toll-like receptor 2 functions as a pattern recognition receptor for diverse bacterial products. J Biol Chem. 1999 Nov 19;274(47):33419-25.

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Pat. No. US 7,071,310

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Unless indicated otherwise, all products are For Research Use Only. Not for use in diagnostic or therapeutic procedures. Not for further distribution without written consent. Not all products available in all regions.