PE anti-human TLR1 (CD281, Toll-like receptor 1, TLR-1) antibody: r-phycoerythin (r-PE) conjugate

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Contents: Phycoerythrin (PE) anti-human Toll-like receptor 1 (CD281, TLR1)
Catalog Number: 12-9911
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: GD2.F4
Isotype: Mouse IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-9911-71	25 tests	$150
12-9911-73	100 tests	$290

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood cells with PE mouse IgG1 isotype control (cat.12-4719) (open histogram) or PE GD2.F4 (colored histogram). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-9911	PE anti-human Toll-like receptor 1 (CD281, TLR1, TLR-1)	488	575	FC
14-9911	Affinity Purified anti-human Toll-like receptor 1 (CD281, TLR1, TLR-1)	N/A	N/A	FC IHC
16-9911	Functional Grade* Purified anti-human Toll-like receptor 1 (CD281, TLR1, TLR-1)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The GD2.F4 antibody reacts with human Toll-like receptor 1 (TLR1). TLR1 is expressed in low numbers on the surface of peripheral blood monocytes and even lower numbers on dendritic cells. A very high degree of donor variability has been reported for TLR1 expression. eBioscience manufactured GD2.F4 mAb has been tested by flow cytometry, in parallel with a sample from the developer using cells from healthy donors. eBioscience and the reference antibody gave similar results: donors tested confirmed low levels expression of TLR1 on PBMC (less than 1/4 log shift). While the ligand and exact function of TLR1 are not defined yet, it has been suggested that TLR1 may cooperate with TLR2 in response to certain antagonists. Despite low levels of surface expression as detected by flow cytometric analysis, TLRs are efficient signal transducing molecules in monocytes and dendritic cells. To date, at least ten members of the Toll family have been identified in human. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The GD2.F4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The GD2.F4 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (1 μg) per million cells in a 100 μl total staining volume.

References

A. Visintin, et al. "Regulation of toll-like receptors in human monocytes and dendritic cells." J. Immunol. 166: 249 (2001).
D.H. Wyllie, et al. "Evidence for an accessory protein function for toll-like receptor 1 in anti-bacterial responses." J. Immunol. 165: 7125 (2000).
Krutzik SR, Ochoa MT, Sieling PA, et al. Activation and regulation of Toll-like receptors 2 and 1 in human leprosy. Nat Med. 2003 May;9(5):525-532.

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