Product Information

Contents: PE anti-human DR5 (CD262, Trail-R2, TrailR2)
Catalog Number: 12-9908
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DJR2-4 (a.k.a. 7-8)
Isotype: Mouse IgG1, κ

Surface staining of human DR5 transfected cells with anti-human DR5 (DJR2-4) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9908	Affinity Purified anti-human DR5 (CD262, Trail-R2, TrailR2)	N/A	N/A	FC
13-9908	Biotin anti-human DR5 (CD262, Trail-R2, TrailR2)	N/A	N/A	FC
12-9908	PE anti-human DR5 (CD262, Trail-R2, TrailR2)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DJR2-4 monoclonal antibody reacts with human DR5, also known as TRAIL-R2, Apo2, TRICK2 and KILLER. DR5 binds to TRAIL, activates NF-κB, and induces TRAIL-mediated apoptosis. DR5 is expressed broadly by normal tissues as well as several tumor cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DJR2-4 (a.k.a. 7-8) antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DJR2-4 (a.k.a. 7-8) antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes. This can be used at 20 μl (0.25 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Earel JK Jr, Vanoosten RL, Griffith TS. Histone deacetylase inhibitors modulate the sensitivity of tumor necrosis factor-related apoptosis-inducing ligand-resistant bladder tumor cells. Cancer Res. 2006 Jan 1;66(1):499-507. (FC, PubMed)

Related Products

Cat. 12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control
Cat. 14-9909 Affinity Purified anti-human DR5 (CD262, Trail-R2, TrailR2) (clone DJR2-2 (a.k.a. 2-6))