mouse/human Fn14 antibody, Phycoerythrin (PE) conjugate

Contents: Phycoerythrin (PE) anti-mouse/human Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14)
Catalog Number: 12-9018
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: ITEM-4
Isotype: Mouse IgG2b, κ

Product Options		Price
Cat. No.	Size	To view relevant pricing, please select your country and resume browsing.
12-9018-73	100 tests
12-9018-80	25 ug
12-9018-82	100 ug

Staining of non-transfected (left) and human Fn14 transfected (right) L5178Y cells with 0.5 µg PE mouse IgG2b iso cntrl (cat.12-4732) (open histogram) or 0.5 µg PE anti-Fn14 (ITEM-4)(closed histogram). Total viable cells were analyzed.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-9018	Affinity Purified anti-human and mouse Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14)	N/A	N/A	FC IH/F WB
13-9018	Biotin anti-human and mouse Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14)	N/A	N/A	FC
12-9018	PE anti-human and mouse Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The ITEM-4 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The ITEM-4 antibody is offered in 2 formats:

- μg size: has been tested by flow cytometric analysis on human or mouse Fn14 transfected cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by flow cytometric analysis on human or mouse Fn14 transfected cells. This can be used at 20 μl per million cells in a 100 μl total staining volume. The pre-titrated test size contains 0.2% BSA.

References

Nakayama, M., K. Ishidoh, Y. Kojima, N. Harada, E. Kominami, K. Okumura, H. Yagita. 2003. Fibroblast Growth Factor-Inducible 14 Mediates Multiple Pathways of TWEAK-Induced Cell Death. The Journal of Immunology, 170: 341-348.
Harada N., M. Nakayama, H. Nakano, Y. Fukuchi, H. Yagita, K. Okumura. 2002.Pro-inflammatory Effect of TWEAK/Fn14 Interaction on Human Umbilical Vein Endothelial Cells. Biochem Biophys Res Commun, 299(3):488-93.
Nakayama M, K. Ishidoh, N. Kayagaki, Y. Kojima, N. Yamaguchi, H. Nakano, E. Kominami, K. Okumura, H. Yagita. 2002. Multiple pathways of TWEAK-induced cell death. J Immunol, 168(2):734-43

Related Products

Cat. 12-4732  PE Mouse IgG2b Isotype Control
Cat. 14-9016  Affinity Purified anti-human and mouse Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14) (clone ITEM-2)
Cat. 16-9016  Functional Grade Purified anti-human and mouse Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14) (clone ITEM-2)
Cat. 12-9019  PE anti-human Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14) (clone ITEM-1)
Cat. 14-9019  Affinity Purified anti-human Fn14/TWEAK Receptor (CD266, TWEAKR, Fn-14) (clone ITEM-1)