Product Information

Contents: Phycoerythrin (PE) anti-mouse/human Granzyme B
Catalog Number: 12-8899
Sizes: 25 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: GB11
Isotype: Mouse IgG1

Left: Human peripheral blood cells were stimulated for 2 days with IL-2. The cells were surface stained with FITC CD56 (MEM188) (cat 11-0569) and stained intracellularly with PE GB11. Quadrants demarcate boundary for isotype controls.
Right: Mouse splenocytes were stimulated for 3 days with IL-2. Cells were surface stained with CD49d (DX5) (cat 17-5971) and subsequently stained intracellularly with PE GB11.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-8899	Phycoerythrin (PE) anti-human Granzyme B	488	575	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The GB11 antibody reacts with human Granzyme B (GrB). GrB is one of the family of serine proteases, known as granzymes, that is located in the granules of cytotoxic T cells and NK cells. Granzyme B has been described also as CGL1 (cathepsin G-like-1), a serine protease expressed only in cytotoxic T-lymphocytes after cell activation. GrB has been called CTLA-1 (cytotoxic T lymphocyte-associated serine esterase 1) based on identification of mRNA in various cytotoxic T cells, but not observed in non-cytotoxic lymphoid cells. GrB is crucial for the rapid induction of target cell death by apoptosis, induced by interaction with cytotoxic T cells. The receptor involved has been identified as mannose 6-phosphate receptor. This receptor functions as a death receptor for granzyme B during cytotoxic T cell-induced apoptosis.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The GB11 antibody has been reported useful for intracellular staining, ELISA, and ELISPOT.

Applications Tested

This GB11 antibody has been pre-titrated and tested on both cultured human PBMCs and mouse splenocytes. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume. It is recommended that the antibody be further titered, particularly when staining mouse tissue. Please refer to the anti-mouse Granzyme B for additional staining data using an alternate antibody, clone 16G6.

References

Trinchieri, G., et al. 1984. Perussia B: Human natural killer cells: biologic and pathologic aspects. Lab Invest. 50: 489-513.
Smyth, M., et al. 1995. Granzymes: exogenous proteinases that induce target cell apoptosis. Immunol Today. 16: 202-206.
Shafer-Weaver, K., et al. 2003. The Granzyme B ELISPOT assay: an alternative to the 51Cr-release assay for monitoring cell-mediated cytotoxicity. J. Translational Med. 1: 14.
Rininsland, F., et al. 2000. Granzyme B ELISPOT assay for ex vivo measurements of T cell immunity. J Immunol Meth. 240:143-155.

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