Contents: Phycoerythrin (PE) anti-mouse/human SSEA-3 (stage-specific embryonic antigen-3)
Catalog Number: 12-8833
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioMC-631 (MC-631)
Isotype: Rat IgM
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Staining of the 2102Ep cell line with PE Rat IgM Iso Cntrl (cat. 12-4341) (open histogram) or PE anti-human SSEA-3 (stage-specific embryonic antigen-3) (colored histogram). Total viable cells were used for analysis.
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Description
The eBioMC-631 (MC-631) monoclonal antibody reacts with the carbohydrate moiety galactosylgloboside on Stage-specific embryonic antigen-3 (SSEA-3). Expression is restricted to the surface of human teratocarcinoma stem cells (EC), human embryonic germ cells (EG) and human embryonic stem cells (ES) and down regulated following differentiation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioMC-631 (MC-631) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioMC-631 (MC-631) antibody has been pre-titrated and tested by flow cytometric analysis of the 2102Ep cell line. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.
References
Andrews PW, Goodfellow PN, Shevinsky LH, Bronson DL, Knowles BB. Cell-surface antigens of a clonal human embryonal carcinoma cell line: morphological and antigenic differentiation in culture.
Int J Cancer. 1982 May 15;29(5):523-31.
Kannagi R, Cochran NA, Ishigami F, Hakomori S, Andrews PW, Knowles BB, Solter D. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells.
EMBO J. 1983;2(12):2355-61.
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12-4341 PE Rat IgM Isotype Control
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12-8843 Phycoerythrin (PE) anti-human SSEA-4 (stage-specific embryonic antigen-4) (clone eBioMC-813-70 (MC-813-70))
Mouse Cellular Antigens Chart
Human Cellular Antigens Chart