Phycoerythrin (PE) anti-human Granulysin

Contents: Phycoerythrin (PE) anti-human Granulysin
Catalog Number: 12-8828
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioDH2 (DH2)
Isotype: Mouse IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-8828-71	25 tests	$100
12-8828-73	100 tests	$240

*International customers: Please contact your distributor for region specific pricing.

Staining of normal human peripheral blood mononuclear cells with FITC anti-human CD56 (MEM188) (cat. 11-0569) then fixed and permeabilized and stained with 0.25 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or PE anti-human Granulysin (right). Cells in the lymphocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-8828	Phycoerythrin (PE) anti-human Granulysin	488	575	IC Flow IH/F

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioDH2 monoclonal antibody reacts with human granulysin, a protein involved in the cytolytic pathway. Granulysin is a member of the saposin-like protein (SAPLIP) family. Two proteins 9 and 15kDa are thought to be post translation modified versions of the 15kDa protein with a change in confirmation. Granulysin is found in NK and activated T cells and the expression is confined to the cytolytic granules which also contains perforin and granzyme. Granulysin with perforn are suspected to function synergistically to kill bacteria and induce apoptosis in nucleated cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioDH2 (DH2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis and immunocytochemistry.

Applications Tested

This eBioDH2 (DH2) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of human peripheral blood cells. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.

References

Gansert JL, Kiessler V, Engele M, Wittke F, Rollinghoff M, Krensky AM, Porcelli SA, Modlin RL, Stenger S. Human NKT cells express granulysin and exhibit antimycobacterial activity. J Immunol. 2003 Mar 15;170(6):3154-61.

Hanson DA, Kaspar AA, Poulain FR, Krensky AM. Biosynthesis of granulysin, a novel cytolytic molecule. Mol Immunol. 1999 May;36(7):413-22.

Ochoa MT, Stenger S, Sieling PA, Thoma-Uszynski S, Sabet S, Cho S, Krensky AM, Rollinghoff M, Nunes Sarno E, Burdick AE, Rea TH, Modlin RL.T-cell release of granulysin contributes to host defense in leprosy. Nat Med. 2001 Feb;7(2):174-9.

Stegelmann F, Bastian M, Swoboda K, Bhat R, Kiessler V, Krensky AM, Roellinghoff M, Modlin RL, Stenger S. Coordinate expression of CC chemokine ligand 5, granulysin, and perforin in CD8+ T cells provides a host defense mechanism against Mycobacterium tuberculosis. J Immunol. 2005 Dec 1;175(11):7474-83.

Stenger S, Hanson DA, Teitelbaum R, Dewan P, Niazi KR, Froelich CJ, Ganz T, Thoma-Uszynski S, Melian A, Bogdan C, Porcelli SA, Bloom BR, Krensky AM, Modlin RL.An antimicrobial activity of cytolytic T cells mediated by granulysin. Science. 1998 Oct 2;282(5386):121-5.

Stenger S, Hanson DA, Teitelbaum R, Dewan P, Niazi KR, Froelich CJ, Ganz T, Thoma-Uszynski S, Melian A, Bogdan C, Porcelli SA, Bloom BR, Krensky AM, Modlin RL. An antimicrobial activity of cytolytic T cells mediated by granulysin. Science. 1998 Oct 2;282(5386):121-5.

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