Product Information

Contents: Phycoerythrin (PE) anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)
Catalog Number: 12-8813
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioMC-480 (MC-480)
Isotype: Mouse IgM

Staining of the F9 cell line with PE Mouse IgM Iso Cntrl (cat. 12-4752) (open histogram) or PE anti-mo/hu SSEA-1 (MC-480) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-8813	Affinity Purified anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)	N/A	N/A	FC IHC IP
13-8813	Biotin anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)	N/A	N/A	FC
12-8813	Phycoerythrin (PE) anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)	488	575	FC
51-8813	Alexa Fluor® 647 anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)	633	668	FC
53-8813	Alexa Fluor® 488 anti-mouse/human SSEA-1 (stage-specific embryonic antigen-1)	488	519	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. SSEA-1 is associated with cell adhesion, migration and differentiation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by flow cytometric analysis of the F9 cell line. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Anjos-Afonso F, Bonnet D. Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartment. Blood. 2007 Feb 1;109(3):1298-306. (PubMed)

Fenderson BA, De Miguel MP, Pyle AD, Donovan PJ. Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens. Methods Mol Biol. 2006;325:207-24. (PubMed)

Solter D, Knowles BB. Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1). Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565-9. (PubMed)

Related Products

Cat. 12-4752 PE Mouse IgM Isotype Control