Staining of the F9 cell line with Mouse IgM Isotype Control PE (cat. 12-4752) (open histogram) or Anti-Human/Mouse SSEA-1 PE (filled histogram). Total viable cells were used for analysis.
Contents: Anti-Human/Mouse SSEA-1 PE Catalog Number: 12-8813 Concentration: 20 μl (0.125 μg)/test Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3 Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material. Clone: eBioMC-480 (MC-480) Host/Isotype: Mouse IgM
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Cat. No.
Size
Price
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12-8813-71
25 tests
12-8813-73
100 tests
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
Description
The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine & human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.
Applications Tested
This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by flow cytometric analysis of the F9 cell line. This can be used at 20 μl (0.125 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.
References
Anjos-Afonso F, Bonnet D. Nonhematopoietic/endothelial SSEA-1+ cells define the most primitive progenitors in the adult murine bone marrow mesenchymal compartment.
Blood. 2007 Feb 1;109(3):1298-306. (PubMed)
Fenderson BA, De Miguel MP, Pyle AD, Donovan PJ. Staining embryonic stem cells using monoclonal antibodies to stage-specific embryonic antigens.
Methods Mol Biol. 2006;325:207-24. (PubMed)
Solter D, Knowles BB. Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1).
Proc Natl Acad Sci U S A. 1978 Nov;75(11):5565-9. (PubMed)
