Product Information

Contents: Phycoerythrin (PE) anti-mouse CMKLR1 (serpentine chemokine-like receptor 1)
Catalog Number: 12-7582
Sizes: 50 ug, 100 ug
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: BZ194 (194)
Isotype: Rat IgG2a, κ

Staining of resident mouse peritoneal macrophages with 0.25 μg of PE Rat IgG2a Iso Cntrl (cat. 12-4321) (open histogram) or 0.25 μg of PE anti-mouse CMKLR1 Chemokine-like receptor 1 (BZ194) (colored histogram). Cells in the large scatter population (CD11b positive) were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7582	Affinity Purified anti- mouse CMKLR1 (serpentine chemokine-like receptor 1)	N/A	N/A	FC
12-7582	Phycoerythrin (PE) anti-mouse CMKLR1 (serpentine chemokine-like receptor 1)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 194.7.4 (BZ194)monoclonal antibody reacts to mouse Chemoattractant-like receptor 1 (CMKLR1), also known as ChemR23 and DEZ. CMKLR1 is the receptor for chemerin, a potent chemoattractant. The BZ194 antibody was generated against the amino terminal residues of mouse CMKLR1. In the human CMKLR is found on pDC while in the mouse expression is confined to resident peritoneal macrophages with highest expression on Cd11b^lo macrophages. Expression is absent on in vitro cultured bone marrow DCs (except on early DCs as defined by CD11c⁺MHCII^int), and on freshly isolated CD11c+ cells from lymph node, spleen and bone marrow. Mouse CMKLR is thought to play a role in macrophage function as a result of injury.

Applications Reported

For research use only, not for diagnostic or therapeutic use. This BZ194 (194) antibody has been reported for use in flow cytometric analysis.

Applications Tested

This BZ194 (194) antibody has been tested by flow cytometric analysis of resident mouse peritoneal macrophages. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Zabel BA, Ohyama T, Zuniga L, Kim JY, Johnston B, Allen SJ, Guido DG, Handel TM, Butcher EC.Chemokine-like receptor 1 expression by macrophages in vivo: regulation by TGF-beta and TLR ligands. Exp Hematol. 2006 Aug;34(8):1106-14. (BZ194, FC, PubMed)

Related Products

Cat. 12-4321 PE Rat IgG2a Isotype Control
Cat. 12-7581 Phycoerythrin (PE) anti-human CMKLR1 (serpentine chemokine-like receptor 1, DC-expressed serpentine receptor, pDC-marker, plasmacytoid DC-marker) (clone BZ332)