Product Information

Contents: Phycoerythrin (PE) anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40
Catalog Number: 12-7235
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBioHP40 (HP40, HP-40)
Isotype: Mouse IgG1, κ

Intracellular staining of unstimulated (left) or IFN-Gammma, LPS, and Monensin stimulated (right) normal human peripheral blood cells with FITC anti-human CD14 (61D3) (cat. 11-0149) and PE anti-human IL-12/23 p40 (eBioHP40). Cells in the monocyte gate were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7235	Phycoerythrin (PE) anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	488	575	IC Flow
51-7235	Alexa Fluor® 647 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	633	668	IC Flow
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBioHP40 antibody reacts with the p40 subunit of human IL-12 p70, and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.

The eBioHP40 antibody has slightly lower background staining than C8.6 and recognizes a different epitope.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The C8.6 antibody has been reported for use in intracellular flow cytometric analysis.

Applications Tested

This eBioHP40 (HP40, HP-40) antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis or stimulated nomral human peripheral blood cells. This can be used at 20 μl (0.06 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Cho, D., et al. 1996. Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 172: 29-34.

Aragane, Y, et al. 1994. IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 153: 5366-72.

Related Products

Cat. 12-4714  Phycoerythrin (PE) Mouse IgG1, K Isotype Control
Cat. 12-7123  PE anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40 (clone C17.8)
Cat. 12-7129  PE anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 (clone C8.6)