Contents: Phycoerythrin (PE) anti-human IL-21 (Interleukin-21, IL21)
Catalog Number: 12-7219
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio3A3-N2 (3A3-N2)
Isotype: Mouse IgG1
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Intracellular staining of human PBMC treated with PMA and Ionomycin in the presence of Brefeldin A for 5 hours with FITC anti-human CD4 (RPA-T4) (cat. 11-0049) and 0.5 μg of PE Mouse IgG1, K Iso Cntrl (cat. 12-4714) (left) or 0.5 μg of PE anti-human IL-21 (3A3-N2) (right). Cells in the lymphocyte gate were used for analysis.
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Description
The monoclonal antibody eBio3A3-N2 reacts with human IL-21, a potent stimulator of NK and cytolytic T cells. IL-21 is a 131 amino acid protein most closely related to IL-2 and IL-15. Expression is found in CD4+ cells upon activation. In addition to its effect on NK cells, IL-21 stimulates proliferation of B-cell stimulated by crosslinking of the CD40 antigen and stimulates the proliferation of bone marrow progenitor cells and the expression of the NK-cell marker CD56 in the presence of IL-15.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This eBio3A3-N2 (3A3-N2) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
This eBio3A3-N2 (3A3-N2) antibody has been pre-titrated and tested by intracellular staining of
stimulated normal human PBMCs. This can be used at 20 μl (0.5 μg) per million cells in a 100 μl total staining volume.
Staining for IL-21 has shown donor variability. Stimulation times may vary depending on the donor and should be optimized. Increased staining levels have been observed on Th17-polarized PBMCs. Please contact Technical support at tech@ebioscience.com for additional information.
References
Parrish-Novak J, et al. Interleukin 21 and its receptor are involved in NK cell expansion and regulation of lymphocyte function.
Nature. 2000 Nov 2;408(6808):57-63.
PubMed.
Parrish-Novak J, Foster DC, Holly RD, Clegg CH.Interleukin-21 and the IL-21 receptor: novel effectors of NK and T cell responses.
J Leukoc Biol. 2002 Nov;72(5):856-63.
PubMed.
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12-4714 Phycoerythrin (PE) Mouse IgG1, K Isotype Control
