Contents: Phycoerythrin (PE) anti-human IL-17A (Interleukin-17A, IL17A)
Catalog Number: 12-7179
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN3, BSA and/or gelatin for protein stabilization
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio64DEC17
Isotype: Mouse IgG1, κ
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Human PBMC's were stimulated with PMA/Ionomycin for 4 hours in the presence of monensin. Cells were harvested and surface stained with FITC anti-human CD3 (clone OKT3, cat.11-0037), then fixed, permeabilized, and stained with PE conjugated eBio64DEC17 antibody.
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Description
The eBio64DEC17 antibody reacts with human IL-17A. The eBio64DEC17 antibody is a neutralizing antibody. Interleukin-17A (IL-17A) is a CD4+ T cell-derived cytokine that promotes inflammatory responses in cell lines and is elevated in rheumatoid arthritis, asthma, multiple sclerosis, psoriasis, and transplant rejection. The cDNA encoding human IL-17A was isolated from a library of CD4+ T cells; the encoded protein exhibits 72 percent amino acid identity with HVS13 , an open reading frame from a T lymphotropic Herpesvirus saimiri, and 63 percent with mouse CTLA-8 (cytotoxic T-lymphocyte associated antigen-8). Human IL-17A exists as glycosylated 20-30 kD homodimers. High levels of IL-17A homodimer are produced by activated peripheral blood CD4+ T-cells. IL-17A enhances expression of the intracellular adhesion molecule-1 (ICAM-1) in human fibroblasts. Human IL-17A also stimulates epithelial, endothelial, or fibroblastic cells to secrete IL-6, IL-8, G-CSF, and PGE2. In the presence of human IL-17A, fibroblasts can sustain the proliferation of CD34+ hematopoietic progenitors and induce maturation into neutrophils. Mouse, rat, and human IL-17A can induce IL-6 secretion in mouse stromal cells, indicating that all homologs can recognize the mouse IL-17A receptor.
IL-23-dependent, IL-17A-producing CD4+ T cells (Th-17 cells) have been identified as a unique subset of Th cells that develops along a pathway that is distinct from the Th1- and Th2- cell differentiation pathways. The hallmark effector molecules of Th1 and Th2 cells, e.g., IFN-g and IL-4, have each been found to negatively regulate the generation of these Th-17 cells.
Intracellular staining by eBio64DEC17 antibody identifies the same cell population as the eBio64CAP17 antibody, as can be seen in co-staining experiments using both antibodies. Click here for link to data
51-7179.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The eBio64DEC17 antibody has been reported for use as the detection antibody in human IL-17A ELISA and ELISPOT assays, as well as for neutralization and intracellular staining.
Applications Tested
This eBio64DEC17 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated PBMCs. This can be used at 20 μl (0.25 μg) per million cells in a 100 μl total staining volume.
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12-7178 Phycoerythrin (PE) anti-human IL-17A (Interleukin-17A, IL17A) (clone eBio64CAP17)
