Contents: Phycoerythrin (PE) anti-human interleukin-12 (IL-12) p40/70
Catalog Number: 12-7129
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: C8.6
Isotype: Mouse IgG1, κ
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Intracellular staining of unstimulated (left) and 2 hour IFNγ LPS overnight (right) normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or PE anti-human IL-12 p40/70 (C8.6) (colored histogram). Cells in the monocyte gate were used for analysis.
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 14-7129 |
Affinity Purified anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
N/A |
N/A |
IC Flow
NU
WB
|
| 16-7129 |
Functional Grade* Purified anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
N/A |
N/A |
NU
|
| 13-7129 |
Biotin anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
N/A |
N/A |
ELISA det
|
| 12-7129 |
PE anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
488 |
575 |
IC Flow
|
| 51-7129 |
Alexa Fluor® 647 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
633 |
668 |
IC Flow
|
| 53-7129 |
Alexa Fluor® 488 anti-human IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40/70 |
488 |
519 |
IC Flow
|
| 48-7129 |
eFluorTM 450 anti-human IL-12 (Interleukin-12) / IL-23 (Interleukin-23) p40/70 (Pacific Blue® replacement) |
405 |
450 |
IC Flow
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The C8.6 antibody reacts with the p40 subunit of human IL-12 p70 and human IL-23. IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and secreted primarily by activated monocytes, macrophages, and dendritic cells. IL-23 is a biologically-related, heterodimeric cytokine composed of disulfide-linked p40 and p19 subunits.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The C8.6 antibody has been reported for use in intracellular flow cytometric analysis.
Applications Tested
This C8.6 antibody is offered in 2 formats:
- μg size: has been tested by intracellular flow cytometric analysis. This can be used at less than or equal to 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested by intracellular flow cytometric analysis. This can be used at 20 μl (0.06 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume). The pre-titrated test size contains BSA and/or gelatin for protein stabilization.
References
Cho, D., et al. 1996. Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 172: 29-34.
Aragane, Y, et al. 1994. IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 153: 5366-72.
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