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Contents: Phycoerythrin (PE) anti-mouse interleukin-12 (IL-12) Catalog Number: 12-7123 Formulation: Sterile Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL. Clone: C17.8 Isotype: Rat IgG2a, κ Prices for This Product* Cat. No. Size Price Qty Action 12-7123-71 25 tests $90 12-7123-81 50 ug $150 12-7123-82 100 ug $260 *International customers: Please contact your distributor for region specific pricing.

Mouse thioglycolate elicited peritoneal cells were stimulated with LPS in the presence of Brefeldin A for 24 hours. The cells were surface stained with FITC anti-mouse CD11b (M1/70) and intracellularly stained with PE anti-mouse IL-12 (C17.8).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-7123	PE anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	488	575	IC Flow
13-7123	Biotin anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	N/A	N/A	ELISA det
16-7123	Functional Grade* Purified anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40	N/A	N/A	IC Flow  IP  NU  WB
45-7123	PerCP-Cy5.5 anti-mouse IL-12 (Interleukin-12) / IL-23 (Interleukin-23) p40	488	690	IC Flow
51-7123	Alexa Fluor® 647 anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23), p40	633	668	IC Flow
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.   Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

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Description

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The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.

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Applications Reported

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For research use only, not for diagnostic or therapeutic use. The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis.

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Applications Tested

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The C17.8 antibody is offered in 2 formats:

- µg size: has been tested by intracellular flow cytometric analysis and can be used at less than or equal to 0.25 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by flow cytometric analysis and can be used at 20 µl per million cells. The pre-titrated test size contains 0.2% BSA.

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References

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Cho D, Lee WJ, Halloran PJ, Trinchieri G, Kim YB.
Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 1996 Aug 25;172(1):29-34.
Aragane Y, Riemann H, Bhardwaj RS, Schwarz A, Sawada Y, Yamada H, Luger TA, Kubin M, Trinchieri G, Schwarz T.
IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 1994 Dec 15;153(12):5366-72.

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