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Phycoerythrin (PE) anti-mouse interleukin-12 (IL-12)
 
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Contents: Phycoerythrin (PE) anti-mouse interleukin-12 (IL-12)
Catalog Number: 12-7123
Formulation: Sterile Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: C17.8
Isotype: Rat IgG2a, κ

Prices for This Product*
Cat. No. Size Price Qty Action
12-7123-71 25 tests $90
12-7123-81 50 ug $150
12-7123-82 100 ug $260
*International customers: Please contact your distributor for region specific pricing.

   
data image 1
Mouse thioglycolate elicited peritoneal cells were stimulated with LPS in the presence of Brefeldin A for 24 hours. The cells were surface stained with FITC anti-mouse CD11b (M1/70) and intracellularly stained with PE anti-mouse IL-12 (C17.8).
Available Formats of This Product
Cat. No. Format Excite
(nm)
Emit
(nm)
Reported Applications
12-7123 PE anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40 488 575 IC Flow 
13-7123 Biotin anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40 N/A N/A ELISA det 
16-7123 Functional Grade* Purified anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23) p40 N/A N/A IC Flow  IP  NU  WB 
45-7123 PerCP-Cy5.5 anti-mouse IL-12 (Interleukin-12) / IL-23 (Interleukin-23) p40 488 690 IC Flow 
51-7123 Alexa Fluor® 647 anti-mouse IL-12 (Interleukin-12, IL12) / IL-23 (Interleukin-23, IL23), p40 633 668 IC Flow 
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.


Description


The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.


Applications Reported


For research use only, not for diagnostic or therapeutic use. The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis.


Applications Tested


The C17.8 antibody is offered in 2 formats:

- µg size: has been tested by intracellular flow cytometric analysis and can be used at less than or equal to 0.25 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

- test size: has been pre-titrated and tested by flow cytometric analysis and can be used at 20 µl per million cells. The pre-titrated test size contains 0.2% BSA.


References



Cho D, Lee WJ, Halloran PJ, Trinchieri G, Kim YB.
Enhancement of porcine natural killer cell activity by recombinant human and murine IL-12. Cell Immunol. 1996 Aug 25;172(1):29-34.
Aragane Y, Riemann H, Bhardwaj RS, Schwarz A, Sawada Y, Yamada H, Luger TA, Kubin M, Trinchieri G, Schwarz T.
IL-12 is expressed and released by human keratinocytes and epidermoid carcinoma cell lines. J Immunol. 1994 Dec 15;153(12):5366-72.


Related Products


Cat. 12-4321    PE Rat IgG2a Isotype Control
Cat. 14-7122    Affinity Purified anti-mouse IL-12 (Interleukin-12, IL12) (clone C18.2)
Cat. 88-7231    Mouse IL-23 (Interleukin-23, IL23) ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Cat. 88-7234    Mouse IL-23 (p19/p40, IL23) ELISA Ready-SET-Go!
Cat. 88-7921    Mouse IL-12 (Interleukin-12, IL12) p70 ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)
Cat. 14-8231    Recombinant Mouse IL-23 (Interleukin-23, IL23)
Cat. 34-8231    Carrier-Free Recombinant Mouse IL-23 (Interleukin-23, IL23)


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