Product Information

Contents: Phycoerythrin (PE) anti-human IL-1a (Interleukin-1 alpha)
Catalog Number: 12-7118
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 364/3B3-14
Isotype: Mouse IgG1

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7118	Affinity Purified anti-human IL-1a (Interleukin-1 alpha, IL1a)	N/A	N/A	ELISA cap IC Flow
11-7118	Fluorescein isothiocyanate (FITC) anti-human IL-1a (Interleukin-1 alpha, IL1a)	488	518	IC Flow

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Description

The 364/3B3-14 antibody reacts with human interleukin-1alpha (IL-1α).

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 364/3B3-14 antibody has been reported for use as capture antibody in a human IL-1α ELISA and for intracellular staining for flow cytometric analysis.

Applications Tested

The 364/3B3-14 antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human IL-1α in combination with the biotinylated 28.9 (13-7115) antibody for detection and recombinant human IL-1α (14-8019) as the standard. A suitable range of concentrations of this antibody for ELISA capture is 1-4 µg/ml. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/ml - 15 pg/ml should be included in each ELISA plate.
The fluorochrome-conjugated 364/3B3-14 antibody is offered in 2 formats:
- µg size: has been tested by intracellular staining for flow cytometric analysis and can be used at less than or equal to 0.5 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested by intracellular staining for flow cytometric analysis and can be used at 20 μl per million cells in a 100 μl total staining volume.

References

Thorpe, R., et al. 1988. Sensitive and specific immunoradiometric assay for human IL-1α. Lymphokine Res. 2: 119-127.
Sassi, A., et al. 2005. Mechanisms of the natural reactivity of lymphocytes from noninfected individuals to membrane-associated leishmania infantum antigens. 174: 3598-3607. (ELISA)
Caricchio, R., et al. 2003. Ultraviolet B radiation-induced cell death: critical role of ultraviolet dose in inflammation and lupus autoantigen redistribution. J. Immunol. 171: 5778-5786. (Intracellular staining).
Garth, L.J., et al. 2001. Dendritic cell activation and cytokine production induced by group B neisseria meningitidis: interleukin-12 production depends on lipopolysaccharide expression in intact bacteria. Infect. Immunity. 69: 4351-4357. (Intracellular staining).

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