PE anti-mouse IL-6 (Interleukin-6) antibody: r-phycoerythin (r-PE) conjugate

Contents: Phycoerythrin (PE) anti-mouse interleukin-6 (IL-6)
Catalog Number: 12-7061
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL
Clone: MP5-20F3
Isotype: Rat IgG1, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-7061-71	25 tests	$90
12-7061-81	50 ug	$120
12-7061-82	100 ug	$220

*International customers: Please contact your distributor for region specific pricing.

Mouse thiogylcolate induced PEC were stimulated with LPS in the presence of Brefeldin A for 6 hours. The cells were surface stained with FITC anti-mouse F4/80 antigen (BM8) and intracellularly stained with PE anti-mouse IL-6 (MP5-20F3).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
11-7061	Fluorescein isothiocyanate (FITC) anti-mouse IL-6 (Interleukin-6, IL6)	488	518	IC Flow
12-7061	PE anti-mouse IL-6 (Interleukin-6, IL6)	488	575	IC Flow
14-7061	Affinity Purified anti-mouse IL-6 (Interleukin-6, IL6)	N/A	N/A	ELISA cap ELISPOT cap NU
16-7061	Functional Grade* Purified anti-mouse IL-6 (Interleukin-6, IL6)	N/A	N/A	ELISPOT cap NU
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The MP5-20F3 antibody reacts with mouse interleukin-6 (IL-6). The MP5-20F3 antibody is a neutralizing antibody. Mouse IL-6 (also known as B Cell Stimulating Factor, BCSF, and BSF-2) is a 21-28 kDa factor produced by a variety of cell types, including monocytes, fibroblasts and endothelial cells. Upon stimulation, macrophages, T, B, mast, and glial cells, eosinophils, keratinocytes and granulocytes also secrete IL-6. This pleiotrophic cytokine influences the antigen-specific immune response, inflammatory response, and the development of neurons, and is a major mediator of the acute phase reaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MP5-20F3 antibody has been reported for use in ELISA capture, ELISPOT capture, neutralization of IL-6 bioactivity, IHC, and intracellular staining for flow cytometric analysis.

Applications Tested

The PE conjugated MP5-20F3 antibody is offered in 2 formats:

- µg size: has been tested by intracellular staining and flow cytometric analysis and can be used at less than or equal to 0.5 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested by intracellular staining for flow cytometric analysis and can be used at 20 μl per million cells in a 100 μl total staining volume. The pre-titrated test size contains 0.2% BSA.

References

Sander, B., I. Hoiden, et al. (1993). "Similar frequencies and kinetics of cytokine producing cells in murine peripheral blood and spleen. Cytokine detection by immunoassay and intracellular immunostaining." J Immunol Methods 166(2): 201-14.
Starnes, H. F., Jr., M. K. Pearce, et al. (1990). "Anti-IL-6 monoclonal antibodies protect against lethal Escherichia coli infection and lethal tumor necrosis factor-alpha challenge in mice" J Immunol 145(12): 4185-91.

Related Products

Cat. 12-4301    PE Rat IgG1 Isotype Control
Cat. 53-7172    Alexa Fluor® 488 anti-mouse IL-17A (Interleukin-17A, IL17A) (clone eBioTC11-18H10.1)
Cat. 17-7321    APC anti-mouse TNFa (Tumor Necrosis Factor alpha, TNF-alpha, TNF-a) (clone MP6-XT22)
Cat. 88-7974    Mouse IL-17 (Interleukin-17, IL17) Tissue Culture ELISA Ready-SET-Go! Kit (with Pre-Coated Plates)