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Anti-Mouse IL-4 PE

Also known as: Interleukin-4, IL4

RUO: For Research Use Only
 
Contents: Anti-Mouse IL-4 PE
Catalog Number: 12-7041
Concentration: μg size: 0.2 mg/ml; test size: 20 μl (0.06 μg)/test
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 2-8°C. Do not freeze. Light sensitive material.
Clone: 11B11
Host/Isotype: Rat IgG1, κ
 
Product Options
Cat. No. Size Price Add Qty to Cart
12-7041-71 25 tests
To view relevant pricing, please select your country and resume browsing.

12-7041-81 50 ug
12-7041-82 100 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
 
Research Related Products
Cat. No.Format
12-4301 Rat IgG1 K Isotype Control PE
24-5093 Anti-Mouse IgD antiserum (Polyclonal)
 

Available Formats
Cat. No.FormatExcite
(nm)
Emit
(nm)
Reported ApplicationsRegulatory Status
12-7041 Anti-Mouse IL-4 PE 488 575 IC Flow RUO
14-7041 Anti-Mouse IL-4 Purified N/A N/A ELISA cap ELISPOT cap RUO
16-7041 Anti-Mouse IL-4 Functional Grade* Purified N/A N/A NU RUO
17-7041 Anti-Mouse IL-4 APC 633 660 IC Flow RUO
51-7041 Anti-Mouse IL-4 Alexa Fluor® 647 (To Be Discontinued. Refer to Alternative Format: APC cat. 17-7041) 633 668 IC Flow RUO
53-7041 Anti-Mouse IL-4 Alexa Fluor® 488 488 519 IC Flow RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg  (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
  Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes:
Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size.
Fluorochrome Replacements: eBioscience is in the process of replacing all Alexa Fluor® 647 conjugated products with eFluor® 660 conjugated products.
 
Description
The 11B11 antibody reacts with mouse interleukin-4 (IL-4), a 14 kD cytokine. The 11B11 antibody is a neutralizing antibody. Mouse IL-4, also known as BCDF, BCGF-1 and BSF-1, is secreted by activated Th2 and natural killer T cells, and to a lesser extent by Th1 and mast cells. IL-4 is species-specific and promotes proliferation and differentiation of B cells. IL-4, in conjunction with other signals such as CD40, induces Ig isotype switching of B cells and the upregulation of surface MHC class II and CD23.
Applications Reported
For research use only, not for diagnostic or therapeutic use. This 11B11 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested
The PE conjugated 11B11 antibody is offered in 2 formats:
- µg size: has been tested by intracellular staining with flow cytometric analysis and can be used at less than or equal to 0.125 μg/million cells in 100 ul staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
- test size: has been pre-titrated and tested by intracellular staining with flow cytometric analysis and can be used at test size: 20 μl (0.06 μg)/per test. A test is defined as the amount (μg)/test of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. The pre-titrated test size contains 0.2% BSA.
References
Ohara, J. and W. E. Paul. 1985. Production of a monoclonal antibody to and molecular characterization of B-cell stimulatory factor-1. Nature. 315: 333-336.
Abrams, J. 1995. Immunoenzymetric assay of mouse and human cytokines using NIP-labeled anti-cytokine antibodies. In Current Protocols in Immunology. A. Kruisbeek eds. Wiley-Interscience, New York. Unit 6.20.1.
Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York.
Finkelman, F.D., and S.C. Morris. 1999. Development of an assay to measure in vivo cytokine production in the mouse. Int. Immunology. 11: 1811-1818.

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