Product Information

Contents: Phycoerythrin (PE) anti-human interleukin-1 receptor antagonist (IL-1RA)
Catalog Number: 12-7015
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: CRM17
Isotype: Mouse IgG1

Human peripheral blood mononuclear cells were stimulated with LPS in the presence of Brefeldin A for 24 hours. (Left) The cells were surface stained with PE anti-human CD14 (61D3) and intracellulary stained with FITC mouse IgG1 isotype control. (Right) The cells were surface stained with PE anti-human CD14 and intracellularly stained with FITC anti-human IL-1RA (CRM17).

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-7015	Affinity Purified anti-human IL-1RA (Interleukin-1 Receptor Antagonist)	N/A	N/A	ELISA cap IC Flow
11-7015	Fluorescein (FITC) anti-human IL-1RA (Interleukin-1 Receptor Antagonist)	488	518	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The CRM17 antibody reacts with human interleukin-1 receptor antagonist (IL-1RA). IL-1RA is produced by macrophages, monocytes, neutrophils, synovial and dermal fibroblasts. The 17 kDa soluble form of IL-1RA (sIL-1RA) is produced by hepatocytes. IL-1RA inhibits the release of both forms of IL-1, the secretion of IL-2, and the expression of IL-2 receptors on the cell surface. IL-1RA also blocks the stimulation of prostaglandin E2 synthesis in synovial cells and the proliferation of thymocytes.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The CRM17 antibody has been reported for use in intracellular flow cytometric analysis.

Applications Tested

The fluorochrome-conjugated CRM17 antibody has been tested by intracellular staining and flow cytometric analysis of LPS-activated, fixed/permeabilized human peripheral blood mononuclear cells. A recommended range of antibody concentrations for optimal staining is 0.05 - 0.5 ug antibody (or 20 ul of prediluted '25 test' reagent) per 1 million cells in 100 ul staining volume. Please see the eBioscience intracellular staining protocol for further information. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

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