PE anti-human TCL1 (T Cell Leukemia/Lymphoma 1) antibody: r-phycoerythin (r-PE) conjugate

Contents: Phycoerythrin (PE) anti-human TCL1 (T Cell Leukemia/Lymphoma 1)
Catalog Number: 12-6699
Formulation: Phosphate buffered saline, pH 7.2, less than or equal to 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eBio1-21 (1-21)
Isotype: Mouse IgG2b, κ

Prices for This Product*
Cat. No.	Size	Price	Qty	Action
12-6699-71	25 tests	$125
12-6699-73	100 tests	$295
12-6699-80	25 ug	$115
12-6699-82	100 ug	$305

*International customers: Please contact your distributor for region specific pricing.

Staining of Daudi cells with 0.125 μg of PE Mouse IgG2b Iso Cntrl (cat. 12-4732) (open histogram) or 0.125 μg of PE anti-human TCL1 (1-21) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6699	Affinity Purified anti-human TCL1 (T Cell Leukemia/Lymphoma 1)	N/A	N/A	IC Flow IHC(Paraffin) IP WB
12-6699	Phycoerythrin (PE) anti-human TCL1 (T Cell Leukemia/Lymphoma 1)	488	575	IC Flow
17-6699	Allophycocyanin (APC) anti-human TCL1 (T Cell Leukemia/Lymphoma 1)	633	660	FC
51-6699	Alexa Fluor® 647 anti-human TCL1 (T Cell Leukemia/Lymphoma 1)	633	668	IC Flow

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The eBio1-21 antibody reacts with human T cell leukemia/lymphoma 1 (TCL1), a 14 kDa proto-oncogene product with a suggested role in intracellular regulation of T cell signalling. TCL1 was identified as the oncogene located at the 14q32.1 chromosome breakpoint region in T-cell prolymphocytic leukemia (T-PLL). In T-PLL, TCL1 is overexpressed as a result of an inversion or a reciprocal translocation, by juxtaposition to the T-cell receptor promoter/enhancer elements. TCL1 binds to the pleckstrin homology domain of Akt (protein kinase B) family proteins, which facilitates Akt dimerization and activity. By increasing Akt activity TCL1 may enhance the serine/threonine phosphorylation of major Akt signaling substrates, such as Ikk complex, mTOR, BAD, p70S6 kinase, FOXO transcription factors, and GSK3β. These substrates regulate cellular differentiation, growth, survival, and metabolism.

Besides its tumorigenic role in T-PLL, TCL1 is normally expressed in the CD3-CD4-CD8- subset of thymic precursors in the T cell lineage, the plasmacytoid subset of dendritic cells, stimulated (not resting) mature T cells, and B cells up to the germinal center stage of maturation. TCL1 is inappropriately expressed by chromosome rearrangements that lead to pre-malignant clonal T cell expansions and mature T cell tumors. TCL1 shows a regulated expression pattern in chronic lymphocytic leukemia (CLL).

Applications Reported

For research use only, not for diagnostic or therapeutic use. This eBio1-21 (1-21) antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested

This eBio1-21 (1-21) antibody has been tested by intracellular staining followed by flow cytometric analysis of Daudi cells. This can be used at less than or equal to 0.25 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume). It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Herling M, Patel KA, Teitell MA, Konopleva M, Ravandi F, Kobayashi R, Jones D. High TCL1 expression and intact T-cell receptor signaling define a hyperproliferative subset of T-cell prolymphocytic leukemia. Blood. 2008 Jan 1;111(1):328-37. Epub 2007 Sep 21. (eBio1-21, IHC, WB PubMed)

Herling, M., et al. 2006. TCL1 shows a regulated expression pattern in chronic lymphocytic leukemia that correlates with molecular subtypes and proliferative state. Leukemia. 20: 280-285. (PubMed)

Hoyer, K., et al. 2005. T cell leukemia-1 modulates TCR signal strength and IFN-g levels through phosphatidylinositol 3-kinase and protein kinase c pathway activation. J. Immunol. 175: 864-873. (PubMed)

Herling, M., et al. 2003. TCL1 expression in plasmacytoid dendritic cells (DC2s) and the related CD4+CD56+ blastic tumors of skin. Blood. 101: 5007-5009. (PubMed)

Takizawa J, Suzuki R, Kuroda H, Utsunomiya A, Kagami Y, Joh T, Aizawa Y, Ueda R, Seto M. Expression of the TCL1 gene at 14q32 in B-cell malignancies but not in adult T-cell leukemia. Jpn J Cancer Res. 1998 Jul;89(7):712-8. (PubMed)

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