Product Information

Contents: Phycoerythrin (PE) anti-human DR4 (CD261, TRAIL Receptor 1, TRAIL-R1, TRAILR1)
Catalog Number: 12-6644
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DJR1
Isotype: Mouse IgG1, κ

Surface staining of human DR4-transfected cells with anti-human DR4 (DJR1) Biotin followed by SA-PE (cat. 12-4317). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-6644	Affinity Purified anti-human DR4 (CD261, TRAIL Receptor 1, TRAIL-R1, TRAILR1)	N/A	N/A	FC IH/F
13-6644	Biotin anti-human DR4 (CD261, TRAIL Receptor 1, TRAIL-R1, TRAILR1)	N/A	N/A	FC
12-6644	PE anti-human DR4 (CD261, TRAIL Receptor 1, TRAIL-R1, TRAILR1)	488	575	FC

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The DJR1 monoclonal antibody reacts with human DR4, also known as TRAIL-R1. DR4 binds to TRAIL, activates NF-κB, and induces TRAIL-mediated apoptosis. DR4 is expressed by a variety of human tumor cells and at very low level on human leukocytes.

Expression has also been found inside the cell on some cell lines (Zhang et al). This has been confirmed with intracellular staining of Jurkat cell line. Please contact tech@ebioscience.com for more information.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DJR1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

This DJR1 antibody has been pre-titrated and tested by flow cytometric analysis of human DR4-transfected cells. This can be used at 20 μl (0.5 μg) per 100 μl blood (or per 1 million cells in 100 μl total staining volume).

References

Ishiyama K, Ohdan H, Ohira M, Mitsuta H, Arihiro K, Asahara T. Difference in cytotoxicity against hepatocellular carcinoma between liver and periphery natural killer cells in humans. Hepatology. 2006 Feb;43(2):362-72. (DJR1, IHC frozen, PubMed)

Earel JK Jr, Vanoosten RL, Griffith TS. Histone deacetylase inhibitors modulate the sensitivity of tumor necrosis factor-related apoptosis-inducing ligand-resistant bladder tumor cells. Cancer Res. 2006 Jan 1;66(1):499-507. (DJR1, FC)

Zhang XD, Franco A, Myers K, Gray C, Nguyen T, Hersey P. Relation of TNF-related apoptosis-inducing ligand (TRAIL) receptor and FLICE-inhibitory protein expression to TRAIL-induced apoptosis of melanoma. Cancer Res. 1999 Jun 1;59(11):2747-53.

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