Contents: Phycoerythrin (PE) anti-mouse IgA Catalog Number: 12-5994 Formulation: Phosphate buffer pH 7.2, 150 mM NaCl, 0.09% NaN3 Clone: 11-44-2 Host/Isotype: Rat IgG1, κ Storage Conditions: Store at 4°C. DO NOT FREEZE. LIGHT-SENSITIVE MATERIAL.
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Cat. No.
Size
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12-5994-81
50 ug
12-5994-82
100 ug
12-5994-83
200 ug
Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.
Description
The 11-44-2 monoclonal antibody reacts with the α heavy chain of mouse IgA. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgM. IgA is expressed as a membrane-bound form as well as a soluble dimer. Fluorochrome conjugated 11-44-2 is used as a detection secondary for mouse IgA.
Applications Reported
For research use only, not for diagnostic or therapeutic use. 11-44-2 has been reported for use in flow cytometric analysis to detect the surface IgA on mouse B cells.
Applications Tested
The fluorochrome conjugated 11-44-2 has been tested by flow cytometric analysis to detect a mouse IgA primary antibody and can be used at less than or equal to 0.2 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
