Contents: Phycoerythrin (PE) anti-mouse IgD
Catalog Number: 12-5993
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 11-26c (11-26)
Isotype: Rat IgG2a, κ
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Description
The 11-26c monoclonal antibody reacts with the δ heavy chain of mouse IgD. It does not react with other classes of mouse immunoglobulin including IgA, IgG or IgM. IgD is expressed by peripheral mature B cells. 11-26c does not activate B cells.
Applications Reported
For research use only, not for diagnostic or therapeutic use. The 11-26c (11-26) antibody has been reported for use in flow cytometric analysis to detect the surface IgD on mouse B cells.
Applications Tested
The 11-26c (11-26) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per 1 million cells in 100 µl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Nitschke L, Kosco MH, Koehler G, and Lamers MC. 1993. Immunoglobulin D-deficient mice can mount normal immune responses to thymus-independent and -dependent antigens. Proc Natl. Acad. Sci. USA. 90:1887-1891.
Ishihara K, Wood Jr. WJ, Wall R, Sakaguchi N, Michnoff C, Tucker PW, and Kincade PW.1993. Multiple B29 containing complexes on murine B lymphocytes. Common and stage-restricted Ig-associated polypeptide chains. J. Immunol. 150: 2253 - 2262.
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Mouse Cellular Antigens Chart