Phycoerythrin (PE) anti-mouse B7-H1 (CD274, B7H1, PDL-1, PD-L1)

RUO: For Research Use Only

mouse B7-H1 antibody, Phycoerythrin (PE) conjugate

Staining of C57Bl/6 splenocytes with FITC anti-mouse CD3e (145-2C11) (cat. 11-0031) and 0.03 μg PE anti-mouse B7-H1 (MIH5). Quadrant lines demarcate Rat IgG2a iso cntrl (cat. 12-4321). Total viable cells were used for analysis.

Contents: Phycoerythrin (PE) anti-mouse B7-H1 (CD274, B7H1, PDL-1, PD-L1)
Catalog Number: 12-5982
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Clone: MIH5
Host/Isotype: Rat IgG2a, λ
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.

Product Options
Cat. No.	Size	Price	Add Qty to Cart
12-5982-81	50 ug	To view relevant pricing, please select your country and resume browsing.
12-5982-82	100 ug
12-5982-83	200 ug

Note: Several countries will continue to be supplied via distributors. Country specific prices may apply.

Research Related Products
Cat. No.	Format
12-4321	PE Rat IgG2a Isotype Control

Available Formats
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications	Regulatory Status
12-5982	PE anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	488	575	FC	RUO
13-5982	Biotin anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FC	RUO
14-5982	Affinity Purified anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FA FC IH/F WB	RUO
16-5982	Functional Grade* Purified anti-mouse B7-H1 (CD274, B7H1, PD-L1, PDL-1, PDL1)	N/A	N/A	FA FC	RUO
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Description

The MIH5 monoclonal antibody reacts with mouse B7-H1, also known as PD-L1. B7-H1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. B7-H1 is expressed on a majority of leukocytes including T, B, NK and DC. B7-H1 is a ligand for PD-1. Interaction of PD-1 with either PD-L1 (B7-H1) or PD-L2 (B7-DC) results in inhibition of T and B cell responses. MIH5 is reported to be a blocking antibody.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MIH5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The MIH5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Sheng H, Wang Y, Jin Y, Zhang Q, Zhang Y, Wang L, Shen B, Yin S, Liu W, Cui L, Li N. A critical role of IFNgamma in priming MSC-mediated suppression of T cell proliferation through up-regulation of B7-H1. Cell Res. 2008 Aug;18(8):846-57. (MIH5, FC, PubMed)

Saudemont A, Jouy N, Hetuin D, Quesnel B. NK cells that are activated by CXCL10 can kill dormant tumor cells that resist CTL-mediated lysis and can express B7-H1 that stimulates T cells. Blood. 2005 Mar 15;105(6):2428-35. (MIH5, FA, PubMed)

Schoop R, Wahl P, Le Hir M, Heemann U, Wang M, Wüthrich RP. Suppressed T-cell activation by IFN-gamma-induced expression of PD-L1 on renal tubular epithelial cells. Nephrol Dial Transplant. 2004 Nov;19(11):2713-20. (MIH5, FA, WB, IHC frozen, PubMed)

Aramaki O, Shirasugi N, Takayama T, Shimazu M, Kitajima M, Ikeda Y, Azuma M, Okumura K, Yagita H, Niimi M. Programmed death-1-programmed death-L1 interaction is essential for induction of regulatory cells by intratracheal delivery of alloantigen. Transplantation. 2004 Jan 15;77(1):6-12.

Kanai T, Totsuka T, Uraushihara K, Makita S, Nakamura T, Koganei K, Fukushima T, Akiba H, Yagita H, Okumura K, Machida U, Iwai H, Azuma M, Chen L, Watanabe M. Blockade of B7-H1 suppresses the development of chronic intestinal inflammation. J Immunol. 2003 Oct 15;171(8):4156-63.

Tsushima F, Iwai H, Otsuki N, Abe M, Hirose S, Yamazaki T, Akiba H, Yagita H, Takahashi Y, Omura K, Okumura K, Azuma M. Preferential contribution of B7-H1 to programmed death-1-mediated regulation of hapten-specific allergic inflammatory responses. Eur J Immunol. 2003 Oct;33(10):2773-82. (MIH5, FA, IHC frozen)

Freeman GJ, Long AJ, Iwai Y, Bourque K, Chernova T, Nishimura H, Fitz LJ, Malenkovich N, Okazaki T, Byrne MC, Horton HF, Fouser L, Carter L, Ling V, Bowman MR, Carreno BM, Collins M, Wood CR, Honjo T. Engagement of the PD-1 immunoinhibitory receptor by a novel B7 family member leads to negative regulation of lymphocyte activation. J Exp Med. 2000 Oct 2;192(7):1027-34.

Dong H, Zhu G, Tamada K, Chen L. B7-H1, a third member of the B7 family, co-stimulates T-cell proliferation and interleukin-10 secretion. Nat Med. 1999 Dec;5(12):1365-9.