Contents: Phycoerythrin (PE) anti-mouse Sca-1 (Ly-6A/E)
Catalog Number: 12-5981
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN3
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT SENSITIVE MATERIAL.
Clone: D7
Isotype: Rat IgG2a, κ
| Prices for This Product* |
| Cat. No. |
Size |
Price |
Qty |
Action |
*International customers: Please contact your distributor for region specific pricing.
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Staining of 3 day ConA-activated (right) or unstimulated (left) BALB/c mouse splenocytes with 0.06 µg of PE D7. Total viable cells were analyzed. Markers were set based on the autofluorescence sample
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| Available Formats of This Product |
| Cat. No. |
Format |
Excite
(nm) |
Emit
(nm) |
Reported Applications |
| 11-5981 |
FITC anti-mouse Ly-6A/E (Sca-1, Sca1) |
488 |
518 |
FC
|
| 12-5981 |
PE anti-mouse Ly-6A/E (Sca-1, Sca1) |
488 |
575 |
FC
|
| 13-5981 |
Biotin anti-mouse Ly-6A/E (Sca-1, Sca1) |
N/A |
N/A |
FC
|
| 14-5981 |
Affinity Purified anti-mouse Ly-6A/E (Sca-1, Sca1) |
N/A |
N/A |
FC
IP
WB
|
| 15-5981 |
PE-Cy5 anti-mouse Ly-6A/E (Sca-1, Sca1) |
488 |
670 |
FC
|
| 16-5981 |
Functional Grade* Purified anti-mouse Ly-6A/E (Sca-1, Sca1) |
N/A |
N/A |
FC
|
| 17-5981 |
APC anti-mouse Ly-6A/E (Sca-1, Sca1) |
633 |
660 |
FC
|
| 25-5981 |
Phycoerythrin-Cy7 (PE-Cy7) anti-mouse Ly-6A/E (Sca-1, Sca1) |
488 |
760 |
FC
|
| 35-5981 |
Phycoerythrin-Cy5.5 (PE-Cy5.5) anti-mouse Ly-6A/E (Sca-1, Sca1) |
488 |
690 |
FC
|
| 45-5981 |
PerCP-Cy5.5 anti-mouse Ly-6A/E (Sca-1, Sca1) |
488 |
690 |
FC
|
| 56-5981 |
Alexa Fluor® 700 anti-mouse Ly-6A/E (Sca-1, Sca1) (APC-Cy5.5 Equivalent) |
633 |
723 |
FC
|
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted).
*Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg.
Purified: Contains azide, not sterile-filtered, and not endotoxin tested. |
Questions? Please consult our answers to frequently asked questions at
http://www.ebioscience.com/faq.
Description
The D7 monoclonal antibody reacts with mouse Sca-1, an 18 kDa member of the Ly-6 family of GPI-linked surface proteins. D7 reacts with both Ly-6E.1 and Ly-6A.2 molecules expressed by mouse hematopoietic stem cells, myeloid population and peripheral T and B cells. Non-activated splenocytes from Ly-6.2 strains including C57BL, SJL, 129, AKR, and others demonstrate higher frequency of D7-positive cells compared to the Ly-6.1 strains such as BALB/c, C3H, NZB and others. Sca-1 is expressed at high levels upon activation regardless of the Ly-6 haplotype. The Ly-6 family is involved in regulation and function of T cell activation. Sca-1 is a major phenotypic marker for mouse hematopoietic progenitor/stem cell subset.
Applications Reported
For research use only, not for diagnostic or therapeutic use. D7 has been reported for use in flow cytometric analysis.
Applications Tested
This D7 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.25 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
References
Ortega, G., P. E. Korty, et al. (1986). "Role of Ly-6 in lymphocyte activation. I. Characterization of a monoclonal antibody to a nonpolymorphic Ly-6 specificity."
J Immunol 137(10): 3240-6.
Malek, T. R., G. Ortega, et al. (1986). "Role of Ly-6 in lymphocyte activation. II. Induction of T cell activation by monoclonal anti-Ly-6 antibodies."
J Exp Med 164(3): 709-22.
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