Product Information

Contents: Phycoerythrin (PE) anti-mouse pan-NK cells (CD49b, α₂ integrin, very late antigen-2)
Catalog Number: 12-5971
Sizes: 10 ug, 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
500 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: DX5
Isotype: Rat IgM, κ

Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 μg of PE anti-mouse CD49b (DX5) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5971	Affinity Purified anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	N/A	N/A	FC
13-5971	Biotin anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	N/A	N/A	FC
11-5971	FITC anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	488	518	FC
12-5971	PE anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	488	575	FC
17-5971	APC anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	633	660	FC
25-5971	Phycoerythrin-Cy7 (PE-Cy7) anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	488	760	FC
36-5971	Functional Grade* Biotin anti-mouse pan-NK cells (CD49b, alpha 2 integrin, very late antigen-2)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The DX5 monoclonal antibody reacts with CD49b, an antigen expressed on a majority of mouse natural killer cells and a subset of T cells. DX5 reacts with all strains of mouse tested including the most commonly used strains, BALB/c, C57BL/6, C3H, CBA, DBA, AKR, SJL and 129. Simultaneous staining of C57BL/6 spleen cells with anti-NK1.1 mAb (PK136) and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5⁺PK136^- and DX5^-PK136⁺ cells.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The DX5 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The DX5 antibody has been tested by flow cytometric analysis of mouse splenocyte suspensions. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Hussell, T. and P. J. Openshaw (1998). "Intracellular IFN-gamma expression in natural killer cells precedes lung CD8+ T cell recruitment during respiratory syncytial virus infection." J Gen Virol 79(Pt 11): 2593-601.
Vos, Q., J. R. Ortaldo, et al. (1998). "Phenotypic and functional characterization of a panel of cytotoxic murine NK cell clones that are heterogeneous in their enhancement of Ig secretion in vitro." Int Immunol 10(8): 1093-101.
Arase, H., T. Saito, et al. (2001). Cutting edge: the mouse NK cell-associated antigen recognized by DX5 moncoclonal antibody is CD49b (alpha(2) integrin, very late antigen-2). J Immunol 167(3): 1141-4.

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