Product Information

Contents: Phycoerythrin (PE) anti-mouse B7S1 (B7H4, B7-H4, B7-S1, B7x)
Catalog Number: 12-5970
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: Clone 9
Isotype: Rat IgG1

Staining of non-transfected (left) and mouse B7-H4-GFP transfected (right) L5178Y cells with 0.25 μg PE Rat IgG1 Iso Cntrl (cat. 12-4301) (open histogram) or 0.25 μg PE anti-mouse B7S1/B7H4 (Clone 9)(colored histogram). Viable cells expressing GFP were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5970	Purified anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x)	N/A	N/A	FC
13-5970	Biotin anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x)	N/A	N/A	FC
12-5970	Phycoerythrin (PE) anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

Custom Product Requests
Need a custom product? Download the Custom Product Request Form and submit completed form to customs@ebioscience.com.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The Clone 9 monoclonal antibody reacts with mouse B7S1, also known as B7H4. B7S1 is a newly discovered member of the B7 family. It is speculated that the costimulatory regulation of T cells by B7S1 is influenced by the activation status of B cells. The ligand for B7S1 has not been identified yet. This antibody is not blocking. Simultaneous double staining of cells with two anti-mouse B7-H4 antibodies, Clone 9 and 188, suggests that epitopes recognized by these mAbs are different and/or there is no steric hindrance when antibodies are used together.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The Clone 9 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The Clone 9 antibody has been tested by flow cytometric analysis of transfected cells. This can be used at less than or equal to 0.5 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Prasad DV, Richards S, Mai XM, Dong C. 2003. B7S1, a novel B7 family member that negatively regulates T cell activation. Immunity. 18(6):863-73.

Related Products

Cat. 12-4301  PE Rat IgG1 Isotype Control
Cat. 12-5972  Phycoerythrin (PE) anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x) (clone 188)
Cat. 13-5972  Biotin anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x) (clone 188)
Cat. 14-5972  Purified anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x) (clone 188)
Cat. 16-5972  Functional Grade Purified anti-mouse B7-H4 (B7H4, B7S1, B7-S1, B7x) (clone 188)