Product Information

Contents: Phycoerythrin (PE) anti-mouse CD95 Ligand (Fas Ligand, CD178)
Catalog Number: 12-5911
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MFL3
Isotype: Armenian Hamster IgG

Surface staining of mouse Fas Ligand transfected cells with 0.5 μg PE anti-mouse CD178 (MFL3). Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5911	Affinity Purified anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FA FC
16-5911	Functional Grade* Purified anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FA FC
13-5911	Biotin anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	N/A	N/A	FC
12-5911	PE anti-mouse CD95 Ligand (Fas Ligand, CD178, FasL)	488	575	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MFL3 monoclonal antibody reacts with mouse Fas (CD95) Ligand, a 40 kDa type II transmembrane glycoprotein. FasL is a member of the TNF family and is expressed by mouse activated T cells. The interaction of FasL with its receptor CD95 induces Fas-mediated killing. It has been reported that the human FasL antigen is cleaved from the surface by matrix metalloproteinases (MMPs), resulting in a 26 kDa soluble form. The degree of sensitivity for the mouse antigen to MMPs has not been reported.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MFL3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The MFL3 antibody has been tested by flow cytometric analysis of mouse FasL transfected cells and activated T cells. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Kayagaki, N., N. Yamaguchi, et al. (1997). "Polymorphism of murine Fas ligand that affects the biological activity." Proc Natl Acad Sci U S A 94(8): 3914-9.
Nakajima, A., H. Hirai, et al. (2000). "Treatment of lupus in NZB/W F1 mice with monoclonal antibody against fas ligand." J Autoimmun 14(2): 151-7.
Kayagaki, N., A. Kawasaki, et al. (1995). "Metalloproteinase-mediated release of human Fas ligand." J Exp Med 182(6): 1777-83.

Related Products

Cat. 12-4888  Phycoerythrin (PE) Armenian Hamster IgG Isotype Control (clone eBio299Arm)
Cat. 14-5912  Affinity Purified anti-mouse/rat CD95 Ligand (Fas Ligand, CD178, FasL) (clone MFL4)
Cat. 16-5912  Functional Grade Purified anti-mouse/rat CD95 Ligand (Fas Ligand, CD178, FasL) (clone MFL4)