Product Information

Contents: PE anti-mouse OX40 Ligand (CD252, OX40L, OX-40L, CD134L, CD134 Ligand)
Catalog Number: 12-5905
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: RM134L
Isotype: Rat IgG2b, κ

Staining of 3-day unstimulated (left) and 3-day stimulated (anti-mouse IgM + anti-mouse CD40) (right) BALB/c splenocytes with 0.25 µg of PE rat IgG2b isotype control (cat.12-4331) (open histogram) or 0.25 µg of PE RM134L (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5905	Affinity Purified anti-mouse OX40 Ligand (CD252, OX40L, OX-40L, CD134L, CD134 Ligand)	N/A	N/A	FA FC IH/F IP
16-5905	Functional Grade* Purified anti-mouse OX40 Ligand (CD252, OX40L, OX-40L, CD134L, CD134 Ligand)	N/A	N/A	FA FC IP
13-5905	Biotin anti-mouse OX40 Ligand (CD252, OX40L, OX-40L, CD134L, CD134 Ligand)	N/A	N/A	FC
12-5905	PE anti-mouse OX40 Ligand (CD252, OX40L, OX-40L, CD134L, CD134 Ligand)	488	575	FA FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

Questions? Please consult our answers to frequently asked questions at http://www.ebioscience.com/faq.

Description

The RM134L monoclonal antibody reacts with mouse OX-40 Ligand, a member of the TNF superfamily. OX-40L is induced on mouse splenic B cells stimulated with a combination of anti-IgM and anti-CD40. Neither resting nor activated mouse T cells express OX-40L. The interaction of OX-40 (CD134) with OX-40L has been implicated in T cell-dependent humoral response. RM134L inhibits the costimulatory activity of mouse OX-40L transfected cells for anti-CD3-stimulated T cell proliferation.

Applications Reported

For research use only, not for diagnostic or therapeutic use. RM134L has been reported for use in flow cytometric analysis.

Applications Tested

The RM134L antibody has been tested by flow cytometric analysis of activated mouse splenocyte suspensions and can be used at less than or equal to 0.5 µg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Akiba, H., H. Oshima, et al. (1999). "CD28-independent costimulation of T cells by OX40 ligand and CD70 on activated B cells." J Immunol 162(12): 7058-66. (blocking)
Akiba, H., Y. Miyahira, et al. (2000). "Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis." J Exp Med 191(2): 375-80.
Tsukada, N., H. Akiba, et al. (2000). "Blockade of CD134 (OX40)-CD134L interaction ameliorates lethal acute graft-versus-host disease in a murine model of allogeneic bone marrow transplantation." Blood 95(7): 2434-9.

Related Products

Cat. 16-0401 Functional Grade Purified anti-mouse CD40 (clone 1C10)
Cat. 12-4031 Phycoerythrin (PE) Rat IgG2b Isotype Control