Product Information

Contents: Phycoerythrin (PE) anti-mouse 4-1BB Ligand (4-1BBL, 41BBL)
Catalog Number: 12-5901
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: TKS-1
Isotype: Rat IgG2a, κ

Staining of 3-day unstimulated (left) and 3 day stimulated (anti-mouse IgM + anti-mouse CD40) (right) BALB/c splenocytes with 0.5 µg PE rat IgG2a iso cntrl (cat.12-4321) (open histogram) or 0.5 µg PE anti-mouse 4-1BB Ligand (TKS-1) (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-5901	PE anti-mouse 4-1BB Ligand (4-1BBL, 41BBL, CD137L, CD137 Ligand)	488	575	FC
13-5901	Biotin anti-mouse 4-1BB Ligand (4-1BBL, 41BBL, CD137L, CD137 Ligand)	N/A	N/A	FC
14-5901	Affinity Purified anti-mouse 4-1BB Ligand (4-1BBL, 41BBL, CD137L, CD137 Ligand)	N/A	N/A	FA FC
16-5901	Functional Grade* Purified anti-mouse 4-1BB Ligand (4-1BBL, 41BBL, CD137L, CD137 Ligand)	N/A	N/A	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.

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Description

The TKS-1 monoclonal antibody reacts with mouse 4-1BB Ligand (4-1BBL), a type II transmembrane protein and a member of the tumor necrosis factor superfamily. 4-1BBL is expressed by activated antigen presenting cells including B cells and macrophages. Interaction of 4-1BBL with 4-1BB (CDw137) is reported to deliver a costimulatory signal important in the T-APC interaction.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The TKS-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The TKS-1 antibody has been tested by flow cytometric analysis of resting and 2-3day (IgM+CD40)-activated mouse splenocytes. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Akiba, H., Y. Miyahira, et al. (2000). "Critical contribution of OX40 ligand to T helper cell type 2 differentiation in experimental leishmaniasis." J Exp Med 191(2): 375-80.
Futagawa, T., H. Akiba, et al. 2002. Expression and function of 4-1BB and 4-1BB ligand on murine dendritic cells. Int Immunol 14(3): 275-86.

Related Products

Cat. 12-4321 PE Rat IgG2a Isotype Control