Product Information

Contents: Phycoerythrin (PE) anti-mouse FcεRIα (FceRI-α, high affinity IgE receptor)
Catalog Number: 12-5898
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MAR-1
Isotype: Armenian Hamster IgG

Staining of MC/9 cells with 0.03 µg of PE Armenian hamster IgG isotype control (cat.12-4888) (open histogram) or 0.03 µg of PE MAR-1 (colored histogram). Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5898	Affinity Purified anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FA FC IH/F IP
16-5898	Functional Grade* Purified anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FA FC
13-5898	Biotin anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	N/A	N/A	FC
11-5898	FITC anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	488	518	FC
12-5898	PE anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	488	575	FC
51-5898	Alexa Fluor® 647 anti-mouse FceRI alpha (FceRIa, FceRI-a, FceRI-alpha, high affinity IgE receptor)	633	668	FC
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MAR-1 monoclonal antibody reacts with the FcεRIα subunit, an IgE-binding subunit lacking signal-transducing ability. FcεRIα is expressed on mast and basophil cells and is up-regulated by the presence of IgE. FcεRIα forms a tetrameric complex with one β and two γ subunits. The β and γ subunits possess immunoreceptor tyrosine-based activation motifs (ITAM). The FcεRI complex plays an important role in triggering IgE-mediated allergic reactions.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MAR-1 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The MAR-1 antibody has been tested by flow cytometric analysis of the MC/9 cell line (a mouse mast cell line). This can be used at less than or equal to 0.06 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Arinobu Y, Iwasaki H, Gurish MF, Mizuno S, Shigematsu H, Ozawa H, Tenen DG, Austen KF, Akashi K. Developmental checkpoints of the basophil/mast cell lineages in adult murine hematopoiesis. Proc Natl Acad Sci U S A. 2005 Dec 13;102(50):18105-10. Epub 2005 Dec 5.(mar-1, FC, PubMed)

Obata K, Mukai K, Tsujimura Y, Ishiwata K, Kawano Y, Minegishi Y, Watanabe N, Karasuyama H. Basophils are essential initiators of a novel type of chronic allergic inflammation. Blood. 2007 Aug 1;110(3):913-20. Epub 2007 Apr 4 (Mar-1, FC)

Sokol CL, Barton GM, Farr AG, Medzhitov R. A mechanism for the initiation of allergen-induced T helper type 2 responses. Nat Immunol. 2008 Mar;9(3):310-8.(Mar-1, FA, PubMed)

Yamaguchi M., K. Hirai, A. Komiya, M. Miyamasu, Y. Furumoto, R. Teshima, K. Ohta, Y. Morita, S. J. Galli, C. Ra, K. Yamamoto. (2001) Regulation of Mouse Mast Cell Surface Fc epsilon RI expression by dexamethasone. Int Immunol 13(7):843-51.

Related Products

Cat. 12-4888 Phycoerythrin (PE) Armenian Hamster IgG Isotype Control (clone eBio299Arm)