Product Information

Contents: Phycoerythrin (PE) anti-mouse BP-1 (BP1)
Catalog Number: 12-5891
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: 6C3
Isotype: Rat IgG2a, κ

Staining of C57Bl/6 bone marrow cells with FITC anti-mo/hu B220 (RA3-6B2) (cat. 11-0452) and 0.5 μg of PE Rat IgG2a Iso Cntrl (cat. 12-4321) (left) or 0.5 μg of PE 6C3 (right). Samples were pre-incubated with purified anti-mouse CD16/32 14-0161. Total viable cells were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5891	Affinity Purified anti-mouse BP-1 (BP1)	N/A	N/A	FC IHC IP
13-5891	Biotin anti-mouse BP-1 (BP1)	N/A	N/A	FC
11-5891	FITC anti-mouse BP-1 (BP1)	488	518	FC
12-5891	PE anti-mouse BP-1 (BP1)	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The 6C3 monoclonal antibody reacts with mouse BP-1, also known as Ly-51 and 6C3 antigen. BP-1 is a 140 kDa type II transmembrane protein that is present on the surface as a disulfide-linked homodimer. This molecule is an enzyme with aminopeptidase A (APA) activity and is expressed by bone marrow stromal cells, thymic cortical epithelial cells and at early stages of mouse B cell development, on pre-B cells. The low level of BP-1 expression on pre-B cells can be upregulated in the presence of IL-7.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The 6C3 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The 6C3 antibody has been tested by flow cytometric analysis of mouse bone marrow cell suspensions. This can be used at less than or equal to 1 μg per million cells in a 100 μl total staining volume. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

References

Pillemer, E., C. Whitlok, I. L. Weissman. 1984. Transformatino-associate proteins in murine B-cell lymphomas that are distinct from Abelosn virus gene products. Proc. Natl. Acad. Sci. 81:4434-4438
Morse III, H. C., G. F. Tidmarsh, K. L. Holmes, T. F. Frederickson, J. N. Hartley, J. H. Pierce, W. Y. Langdon, M. O. Dailey, and I. L. Weissman. 1987. Expression of the 6C3 antigen on murine hematopoietic neoplasms. Association with expression of abl, ras, fes, src, erbB, and Cas NS-1 oncogenes but not with myc. J. Exp. Med. 165:920-925
Wu, Q., G. F. Tidmarsh, P. A. Welch, J. H. Pierce, I. L. Weissman, M. D. Cooper. 1989. The early B linage antigen BP-1 and the transformation-associated antigen 6C3 are on the same molecule. J. Immunol. 143:3303-3308

Related Products

Cat. 12-4321  PE Rat IgG2a Isotype Control
Cat. 11-5995  FITC anti-mouse BP-1 (BP1) (clone FG35.4)
Cat. 12-5995  PE anti-mouse BP-1 (BP1) (clone FG35.4)
Cat. 13-5995  Biotin anti-mouse BP-1 (BP1) (clone FG35.4)
Cat. 14-5995  Affinity Purified anti-mouse BP-1 (BP1) (clone FG35.4)