Product Information

Contents: PE anti-mouse IgM
Catalog Number: 12-5890
Sizes: 50 ug, 100 ug, 200 ug
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.1% NaN₃
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: eB121-15F9
Isotype: Rat IgG2a, κ

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
14-5890	Affinity Purified anti-mouse IgM	N/A	N/A	FC
13-5890	Biotin anti-mouse IgM	N/A	N/A	FC
11-5890	FITC anti-mouse IgM	488	518	FC
12-5890	PE anti-mouse IgM	488	575	FC
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.

Applications Reported

For research use only, not for diagnostic or therapeutic use. eB121-15F9 has been reported for use in flow cytometric analysis to detect the surface IgM on mouse B cells and as a second step to detect primary mouse IgMs.

Applications Tested

The eB121-15F9 antibody has been tested by flow cytometric analysis to detect the surface IgM on mouse B cells and can be used at less than or equal to 0.125 μg/million cells. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Related Products

Cat. 12-0990  PE anti-rat IgM (clone HIS40)
Cat. 12-4321  PE Rat IgG2a Isotype Control
Cat. 12-4341  PE Rat IgM Isotype Control
Cat. 12-4752  PE Mouse IgM Isotype Control
Cat. 12-5790  Phycoerythrin (PE) anti-mouse IgM (clone II/41)