Product Information

Contents: Phycoerythrin (PE) anti-human B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)
Catalog Number: 12-5889
Sizes: 25 tests, 100 tests
Formulation: Phosphate buffer pH 7.2,
150 mM NaCl, 0.09% NaN₃, 0.2% BSA
Storage Conditions: Store at 4°C.
DO NOT FREEZE.
LIGHT-SENSITIVE MATERIAL.
Clone: MIH12
Isotype: Mouse IgG1, κ

Surface staining of normal human peripheral blood cells with anti-human B7RP-1 (MIH12) PE. Appropriate isotype controls were used (open histogram). Cells in the lymphocyte population were used for analysis.

Available Formats of This Product
Cat. No.	Format	Excite (nm)	Emit (nm)	Reported Applications
12-5889	PE anti-human B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)	488	575	FC
13-5889	Biotin anti-human B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)	N/A	N/A	FC IH/F
14-5889	Affinity Purified anti-human B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)	N/A	N/A	FA FC IH/F
16-5889	Functional Grade* Purified anti-human B7RP-1 (CD275, B7-RP1, B7RP1, ICOS-L, ICOSL, B7h, GL50, B7-H2, B7H2)	N/A	N/A	FA FC IH/F
*Functional Grade™ (FG™) Purified: Azide-free, sterile-filtered, and endotoxin < 0.001 ng/µg (unless otherwise noted). *Functional Grade™ (FG™) Biotin: Azide-free, sterile-filtered, and endotoxin < 0.05 ng/µg. Purified: Contains azide, not sterile-filtered, and not endotoxin tested.
Flow Cytometry Product Notes: Test Sizes: To accommodate multicolor flow cytometry, eBioscience is in the process of reducing test size volumes from 20 µl to 5 µl. Please check your antibody vial for the recommended test size. Fluorochrome Replacements: eBioscience is in the process of replacing all Pacific Blue® and APC-Alexa Fluor® 750 conjugated products with eFluor™ 450 and APC-eFluor™ 780 conjugated products, respectively.

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Description

The MIH12 monoclonal antibody reacts with human B7RP-1, also known as B7h, B7-H2, GL50 and ICOS Ligand. B7RP-1, a member of the B7 family, has a predicted molecular weight of approximately 40 kDa and belongs to the Ig superfamily. Human B7RP-1 is expressed by activated monocytes/macrophages. B7RP-1 binds to the ICOS molecule (AILIM, CRP-1) expressed by activated T cells. The interaction of ICOS/B7RP-1 plays an important role in the T cell costimulation pathway.

Applications Reported

For research use only, not for diagnostic or therapeutic use. The MIH12 antibody has been reported for use in flow cytometric analysis.

Applications Tested

The MIH12 antibody has been pre-titrated and tested by flow cytometric analysis of human peripheral blood leukocytes and IFN-γ-activated PBMC. This can be used at 20 μl (0.125 μg) per million cells in a 100 μl total staining volume.

References

Youngnak-Piboonratanakit, P., F. Tsushima, et al. 2006. Expression and Regulation of Human CD275 on Endothelial Cells in Healthy and Inflamed Mucosal Tissues. Scand J Immunol 63: 191-198. (MIH12, FC, FA, IH/F, PubMed)
Carreno, B. M. and M. Collins 2002. THE B7 FAMILY OF LIGANDS AND ITS RECEPTORS: New Pathways for Costimulation and Inhibition of Immune Responses. Annu Rev Immunol 20: 29-53.
Liu, X., X. F. Bai, et al. 2001. B7H costimulates clonal expansion of, and cognate destruction of tumor cells by, CD8(+) T lymphocytes in vivo. J Exp Med 194(9): 1339-48.
Iwai, H., Y. Kozono, et al. 2002. Amelioration of collagen-induced arthritis by blockade of inducible costimulator-b7 homologous protein costimulation. J Immunol 169(8): 4332-9.

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